* The trichloroacetic acid extracts were first acidified with sulfuric acid3 and kept overnight at 38” for decarboxylation of acetoacetate. The fluid was then neutralized with sodium hydroxide and phosphate, and the acetone distilled by the procedure of Folin.4 Acetone was determined calorimetrically by the method of Behre.6 Analogous results were obtained with the determination procedure of Le...

One of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance the catalyzed chemical transformation. In this study, dihydrofolate reductase, a small monomeric protein that catalyzes a single C-H-C transfer, is used as a model system to address this question. Experimental and computational studies have proposed a dynamic network that includes two residue...

organophosphorus compounds are highly toxic chemicals that have application in production of warfare agents and agricultural pesticides. detoxification of areas that have been exposed to these types of materials is of vital importance and several approaches have been used for this purpose. biodegradation of organophosphates is one of these approaches that is in the center of attention due to it...

In our previous studies, Salacia extracts demonstrated beneficial effects on the enteric environment of the rat, as represented by decrease in ammonia and other products decomposed by enteric microorganism. In the present study, we showed that the expression of immunologically relevant genes increased in the epithelium of the small intestine from the rat orally dosed with Salacia extracts. T-RF...

Erroneously high values for serum triglyceride levels obtained with the semiautomated method of Lofland were found to be due to contamination of the isopropanol extracts with glucose or other carbohydrate. Treatment of the isopropanol extracts with a mixture of copper sulfate and calcium hydroxide removed the contaminating glucose. Analysis of the glucose-free extracts by either the semiautomat...

The present study evaluated the in vitro anticataract and antioxidant activities of ethanolic seed extract of Abrus precatorius Linn. (Fabaceae) against calcium-induced cataractogenesis using goat lenses. Transparent isolated goat lenes were incubated in artificial aqueous humor and divided into seven experimental groups. The extracts at a dose of 100 μg/ml and 200 μg/ml were incubated simultan...