density-gradient centrifugation. ISCO fractionator scanning patterns indicated a high degree of purity but serological results revealed the presence of some host contaminates. The yield of virus was abeut 2mgfleOg of bro'ad bean leaves. The titer of the antiserum against isolate No. 30 was 1!2048 by ring precipitin tests. Although the iutact virus particles could n6t diffuse in agar gel plates,...

Gerbera spp., which is in the Asteraceae family, most widely cultivated cut flower ornamental plant Türkiye after carnation. Approximately 74% of Türkiye's production met from Antalya province. Virus diseases are a problem cultivation as they significantly reduce yield and quality. In this study, was carried out to detect some virus seen areas province, surveys were commercial 2021-2022 season....

In this study, the real-time PCR assays were combined with high resolution melting (HRM) analysis for the simultaneous detection of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) infection in sweet cherry trees. Detection of CNRMV and CGRMV was performed in a real-time PCR using a primer set for both of them or duplex real-time PCR that included one specif...

Tomato black ring virus (TBRV) and beet ringspot (BRSV) are closely related but distinct members of subgroup B the genus Nepovirus. Both viruses have broad host ranges transmitted by seed, pollen, ectoparasitic nematodes. Although 13 TBRV 3 BRSV genome sequences were already available, no attempt has been made to link sequence data from these recent with those historical isolates studied in pre...

A total of 1,800 blood specimens (1,000 from healthy blood donors, 300 from patients with sexually transmitted diseases, and 500 from intravenous drug users) were simultaneously tested with anti-human immunodeficiency virus enzyme-linked immunosorbent assay (ELISA) kits and a newly developed 2-min test for anti-human immunodeficiency virus based on the principle of autologous erythrocyte agglut...

In this study, we validated three commercial ELISA (NSP-ELISA) kits that detect antibodies to a nonstructural protein of foot-and-mouth disease virus (FMDV) in terms of their specificities and sensitivities. Although the specificities of the NSP-ELISA kits were as high as that of liquid-phase blocking ELISA (LPBE) in non-infected, non-vaccinated animals, the sensitivities of the NSP-ELISA kits ...