Transcription activator-like (TAL) effectors are repeat-containing proteins used by plant pathogenic bacteria to manipulate host gene expression. Repeats are polymorphic and individually specify single nucleotides in the DNA target, with some degeneracy. A TAL effector-nucleotide binding code that links repeat type to specified nucleotide enables prediction of genomic binding sites for TAL effe...

The present paper investigates an intuitive way of robot path planning, called robot teaching by demonstration. In this method, an operator holds the robot end-effector and moves it through a number of positions and orientations in order to teach it a desired task. The presented control architecture applies impedance control in such a way that the end-effector follows the operator’s hand with d...

background: polysaccharides have long been used as immune-modulators in various pathologic conditions including inflammation and solid malignancies. objective: to evaluate the effects of zymosan and betaglucan on cytotoxic reactions in an effectortarget conjugate system. methods: blood was obtained from 20 healthy subjects; purified mononuclear leukocytes (monocytes and lymphocytes) were extrac...

Precise discrimination between similar cellular states is essential for autonomous decision-making scenarios, such as in vivo targeting of diseased cells. Discrimination could be achieved by delivering an effector gene expressed under a highly active context-specific promoter. Yet, a single-promoter approach has linear response and offers limited control of specificity and efficacy. Here, we co...

Plant pathogens secrete effector molecules that contribute to the establishment of disease in their plant hosts. The identification of cellular cues that regulate effector gene expression is an important aspect of understanding the infection process. Nutritional status in the cell has been postulated to be a cue for effector gene expression. Several studies have shown the induction of the same ...

Bacterial leaf streak of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is an increasingly important yield constraint in this staple crop. A mesophyll colonizer, Xoc differs from X. oryzae pv. oryzae (Xoo), which invades xylem to cause bacterial blight of rice. Both produce multiple distinct TAL effectors, type III-delivered proteins that transactivate effector-specific host genes. A TA...

Novel ADCC effector cells expressing the V-variant or F-variant of FcγRIIIa (CD16a) and firefly luciferase under the control of a chimeric promoter incorporating recognition sequences for the principal transcription factors involved in FcγRIIIa signal transduction, together with novel target cells overexpressing a constant high level of the specific antigen recognized by rituximab, trastuzumab,...

background: cd8+ cytotoxic t lymphocytes have been recently divided based on their cytokine expression profile. objective: to evaluate the percentages of cd8+ lymphocytes and their effector subsets including tc1, tc2 and tc17 in the tumor draining lymph nodes (tdlns) of patients with breast cancer. methods: single cell suspensions were obtained from tdlns of 42 patients with breast cancer. stai...

The population of regulatory T cells (Tregs) is critical for immunological self-tolerance and homeostasis. Proper ion regulation contributes to Treg lineage identity, regulation, effector function. Identified channels include Ca2+ release-activated Ca2+, transient receptor potential, P2X, volume-regulated anion K+ Kv1.3 KCa3.1. Ion channel modulation represents a promising therapeutic approach ...

Abstract Long-term protection against infection and disease requires long-lived memory CD8+ T cells. The development of these cells a differentiation process influenced by cytokines – such as IL-2, IL-7, IL-15 that are known to mediate the balance between effector populations. Interestingly, act through common activation STAT5, however, mechanisms which STAT5 dictates cell fate is poorly unders...