Background: Acrylamid (AA) is found in carbohydraterich foods that are prepared at high temperatures, such as French fries and potato chips. The toxic effects of AA in male animals include degeneration of epithelial cells of seminiferous tubules, decreased number of sperm and elevation in abnormal spermatozoa. The aim of this study was to evaluate the detrimental effects of AA on sperm paramete...

Background: Semen cryopreservation is the most important section of artificial insemination programs; it allows preservation of semen fertility for a long time. Materials and Methods: The aim of the present study was to detect the effect of in vitro supplementation of 4-hydroxy Tempo on fresh and frozen spermatozoa quality of buffalo bulls. Five healthy buffalo bulls (5 ejaculates from each bul...

Background: Chronic ethanol abuse causes reproductive organ failure and infertility in both humans and laboratory animals. Since sperm has a critical role in reproductive function, the objective of this unique study was to evaluate the effects of different doses of ethanol on sperm parameters, chromatin structure and apoptosis in adult mice. Materials and Methods: A total of 36 adult male mice ...

Background: Low uptake of exogenous DNA by sperm and reduced number of fertilized oocyte by transfected sperm are the major obstacles for progression of sperm mediated gene transfer. Therefore, the modification of sperm mediated gene transfer procedure needs to be required. The purpose of this study was to evaluate the efficiency of FuGene 6 compare to lipofection in transfection medium for int...

Manufactured nanoparticles have a wide range of application due to their advantageous properties whencompared with their bulk forms. There is a growing concern regarding the safety of nanoparticles. This studyaimed to evaluate the cell membrane lipoperoxidation (LPO) and DNA integrity in red earthworm Lumbricusrubellus when exposed to artificial soil systems containing different concentration o...

Background: Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa. Also, decreased telomere length in spermatozoa of infertile men is associated with the reduction of fertility potential and embryo quality. Density gradient centrifugation (DGC)...

Canine seminal plasma contains antioxidant enzymes to protect sperm against internally generated ROS. These enzymes are removed from seminal plasma during the process of cryopreservation. The freezing/thawing process can cause some morphological and functional changes via ice crystallization and osmolality imbalance. The present study was conducted to evaluate the effects of curcumin supplement...

Real-time quantitative PCR (qPCR) is an indispensable molecular biology tool in the post-genome era. QPCR assays have become a robust and ideal tool for several applications such as clinical diagnostics, gene expression studies, biomarker discovery, and more. Using an intact genomic DNA (gDNA) template is crucial for generating meaningful qPCR data. Therefore, assessment of the gDNA integrity i...

Antioxidants partially ameliorated the negative effects of reactive oxygen species (ROS) produced during cryopreservation. The objective of the present study was to investigate the effect of reduced glutathione, ascorbic acid and α-tocopherol on the quality of frozen-thawed buck spermatozoa. Ejaculates were collected by artificial vagina from 8 bucks and microscopically evaluated at 37 0 C. The...

OBJECTIVES The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. MATERIAL AND METHODS The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was a...