BACKGROUND: The conventional solution-phase Chemical Cleavage of Mismatch (CCM) method is time-consuming, as the protocol requires purification of DNA after each reaction step. This paper describes a new version of CCM to overcome this problem by immobilizing DNA on silica solid supports. RESULTS: DNA test samples were loaded on to silica beads and the DNA bound to the solid supports underwent ...

Using the coupled cluster method ~CCM!, we present a simple accurate calculation for the energies of the groundand first excited states of the linear E ^ e Jahn–Teller and pseudo-Jahn–Teller Hamiltonians. From the solution of a single transcendental equation, we obtain results with a maximal error of 1.2%. These results are notably better than previous results obtained both via the CCM and othe...

Application of electrical field to the heart during the refractory period of the beat has been shown to increase the force of contraction both in animal models and in heart failure patients (cardiac contractility modulation, or CCM). A direct increase in intracellular calcium during CCM has been suggested to be the mechanism behind the positive inotropic effect of CCM. We studied the effect of ...

Cerebral cavernous malformations (CCMs) are vascular lesions affecting the central nervous system. CCM occurs either sporadically or in an inherited, autosomal dominant manner. Constitutional (germline) mutations in any of three genes, KRIT1, CCM2 and PDCD10, can cause the inherited form. Analysis of CCM lesions from inherited cases revealed biallelic somatic mutations, indicating that CCM foll...

‎in this paper‎, ‎we present a new modification of chebyshev-halley‎ ‎method‎, ‎free from second derivatives‎, ‎to solve nonlinear equations‎. ‎the convergence analysis shows that our modification is third-order‎ ‎convergent‎. ‎every iteration of this method requires one function and‎ ‎two first derivative evaluations‎. ‎so‎, ‎its efficiency index is‎ ‎$3^{1/3}=1.442$ that is better than that o...

‎the main purpose of this paper is to study the numerical solution of nonlinear volterra integral equations with constant delays, based on the multistep collocation method. these methods for approximating the solution in each subinterval are obtained by fixed number of previous steps and fixed number of collocation points in current and next subintervals. also, we analyze the convergence of the...

in this paper, elzaki homotopy perturbation method is employed for solving linear and nonlinear differential equations with a variable coffecient. this method is a combination of elzaki transform and homotopy perturbation method. the aim of using elzaki transform is to overcome the deficiencies that mainly caused by unsatised conditions in some semi-analytical methods such as homotopy perturbat...

A method for solving large-scale constraint satisfaction problems is proposed in the present paper. This method is stochastic (or randomized) and uses local information only, i.e., no global plan is expressed in the program and the computation refer to no global information. This method uses CCM (Chemical Casting Model) as a basis, which is a model for emergent computation proposed by the autho...

A modified application of the chemical cleavage of mismatch (CCM) method has been used to screen three contiguous exons (exons 9, 10, and 11) of the phenylalanine hydroxylase gene in 17 Italian PKU patients. A new nonsense heterozygous C-->G transversion within exon 11 (S359X) was identified in a single patient. Only one of the four mutations previously reported in this DNA region in Caucasians...