We fabricated a two-dimensional (2-D) photonic crystal lectin sensing material that utilizes light diffraction from a 2-D colloidal array attached to the surface of a hydrogel that contains mannose carbohydrate groups. Lectin-carbohydrate interactions create hydrogel cross-links that shrink the hydrogel volume and decrease the 2-D particle spacing. This mannose containing 2-D photonic crystal s...

Weekly transfusions of whole blood from a nondiabetic subline of BB/W rats reduced the incidence of diabetes in susceptible BB/W rats from 39 to 0 percent and the incidence of pancreatic insulitis from 64 to 6 percent. Responsiveness of lymphocytes to concanavalin A was found to be low in rats with diabetes or insulitis. Transfusion restored concanavalin A responsiveness to levels observed in c...

Glucocorticoid-resistant P1798 cell lines have been found to contain levels of glucocorticoid receptor comparable to receptor levels in glucocorticoid-sensitive P1798 cells. Pre viously, most of the P1798 resistant cells examined were found to contain low levels of glucocorticoid receptor, and this was thought to account for the resistance of these cells to glucocorticoid treatment. Resistant c...

To assess acute-phase proteins in relation to ageing, we measured serum concentrations of C-reactive protein of AGP in 131 healthy elderly individuals (aged >/= 65 years) living independently in the community, and 47 healthy younger individuals. Concentrations of CRP in the older persons (median = 3.0 microg/ml) were significantly greater than in the younger group (median = 0.9 microg/ml, p = 0...

Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was charact...

from the fermentation broth of Aspergillus candidus RF-5672 (FERM BP-5882), which was isolated from a soil sample collected on Shodo Island, Kagawa Prefecture, Japan. In this communication, we report the fermentation, isolation, structure elucidation and biological properties of these terprenins. The activities of the terprenins found were evaluated with respect to proliferation of mouse spleen...

\Ve llave determined the crystal structure of demetallized concanavalin A, at a resolution of 3.2 8, by rnolecnlar replacement using the knowd structure of native concanavalin A. Refinement of the initial model using a, constraint-restraint reciprocal-space least-squares procedure caused the conventional crystallographic agreement (11) factor t,o decrcase from 0.47 to a final valne of 0.26. The...

When the Mn(2+) and Ca(2+) ions normally present in concanavalin A are removed, the protein becomes incapable of binding saccharides. To explore the structural differences between the native and demetallized forms and their effects on the saccharide-binding properties of the protein, we have refined and compared the crystal structures of both forms. Refinement, carried out by automated differen...

Concanavalin A activates T lymphocytes in vitro and causes T-cell-dependent hepatic injury in mice. T lymphocytes were previously identified as effector cells of concanavalin A-induced liver injury. Here we report that hepatic injury is characterized by apoptotic cell death. On concanavalin A challenge, the cytokines tumor necrosis factor-alpha (TNF alpha), interleukin-2, granulocyte macrophage...