DNA-STR analysis is widely used in the forensic science field and has important requirements on the analysis time to obtain faster inspections. The developed forensic STR kit, referred to as Expressmarker 16 (EX16), could shorten the amplification time to a minimum of 35min. It enables 16 STR loci to be co-detected, including 13 CODIS loci, D2S1338, D6S1043 and Amelogenin loci. The kit is valid...

This paper describes a developmental validation study of three Miniplex sets covering 12 of the 13 CODIS loci. As these new sets will be used for the analysis of degraded and low level DNA, the validation studies were performed using 100-125 pg of DNA, the lowest input level at which peak balance, peak intensity, and allele consistency were stable. To demonstrate the applicability of the Minipl...

Since its development in the mid 1980s, DNA analysis has become a standard procedure utilized by law enforcement and legal systems in the forensic examination of human remains, and to help establish or exclude a connection to a crime scene. The recent progression of gene amplification and enrichment strategies, together with next generation sequencing techniques, have made the analysis of ancie...

Forensic Short Tandem Repeat (STR) genotyping is almost exclusively performed by capillary electrophoresis (CE) in specialized laboratories. As an alternative to CE, and enable miniaturized lab-on-a-chip STR profiling, we developed the QueSTR probes, a hybridization-based assay that relies on recognition cleavage of RNA:DNA duplex RNase H2 enzyme. For each allele be genotyped, matching DNA prob...

In this work, we propose a novel methodology to identify parameters of compartmental epidemiological models. It is based on solving multi-objective optimization problem that consists in fitting some the model outputs real observations. First, according available data considered epidemic, define where are variables. Then, solved by considering particular algorithm called ParWASF-GA (Parallel Wei...

TAIGA (Twente Advanced Interactive Graphic Authoring system) is a system which can be used to develop instructional software. It is written in MS-PASCAL, and runs on computers that support MS-DOS. Desis NI to support the production of structured software, TAIGA has a hierarchical structure of three layers, each with a specific function, and each layer is supported by an editor. Level 1 involves...