The 3'-terminal nucleotide sequence of fibroin mRNA has been determined. A cDNA transcript primed by oligo(dT) hybridized to the poly(A) segment of fibroin mRNA was used to determine the sequence immediately adjacent to poly(A). A specific primer complementary to this sequence then allowed synthesis of 5'-terminally homogeneous transcripts amenable to analysis by rapid sequencing methods. The 3...

Non-coding transcripts play an important role in gene expression regulation in all species, including budding and fission yeast. Such regulatory transcripts include intergenic ncRNA (non-coding RNA), 5' and 3' UTRs, introns and antisense transcripts. In the present review, we discuss advantages and limitations of recently developed sequencing techniques, such as ESTs, DNA microarrays, RNA-Seq (...

Wounding of plants leads to endogenous rise of jasmonic acid (JA) accompanied with the expression of a distinct set of genes. Among them are those coding for the allene oxide cyclase (AOC) that catalyses a regulatory step in JA biosynthesis, and for 1-deoxy-D-xylulose 5-phosphate synthase 2 (DXS2), an enzyme involved in isoprenoid biosynthesis. To address the question how roots and shoots of Me...

Several lines of evidence indicate that the processes of mRNA turnover and translation are intimately linked and that understanding this relationship is critical to elucidating the mechanism of mRNA decay. One clear example of this relationship is the observation that nonsense mutations can accelerate the decay of mRNAs in a process that we term nonsense-mediated mRNA decay. The experiments des...

The connexin32 (cx32) gene codes for the gap junction protein found in liver, pancreas and nervous tissue. Recently mutations in the coding region of this gene have been associated with the dominant X-linked form of Charcot-Marie-Tooth (CMTX1) neuropathy. Since some CMTX1 patients sho,* no mutations in their cx32 gene coding region, it was speculated that these patients carry mutations in the p...

Abstract The neurotrophin Brain-derived neurotrophic factor (BDNF) is encoded by multiple bipartite transcripts. Each BDNF transcript composed one out of 11 alternatively spliced exons containing the 5′untranslated region (UTR), and common exon encompassing coding sequence (CDS) 3′UTR with two variants (short long). In neurons, mRNA have a distinct subcellular distribution, constituting “spatia...

Background: Nowadays long non-coding RNAs are known as interesting functional part of the transcriptome. LncRNA SNHG6 was reported to be expressed more in breast cancer tissues than non-tumor ones. As a frequent cancer among women, breast cancer treatment needs applied biomarkers for fast prognosis and diagnosis. SNHG6 RNA and its splice variants could be considered as molecula...

The human gene coding for the 70-kD polypeptide of the complement regulatory component C4b-binding protein (C4BP alpha) spans over 40 kb of DNA and is composed of twelve exons. Upon transcription in liver, or in Hep-G2 cells, this gene produces a single transcript of 2,262 nucleotides, excepting the poly A tail, that presents an unusually long 5' untranslated region (5' UTR) of 223 nucleotides....