A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on S...

Four cecum and right ventral colon-fistulated horses were assigned in a 4 x 4 Latin square design and fed a high-fiber (HF) or a high-starch (HS) diet with or without 10 g of Saccharomyces cerevisiae (SC; CBS 493.94) containing 4.5 x 10(9) cfu/g. The HF and HS diets consisted of pelleted feeds and long wheat straw (18.0 and 3.5 g of DM.kg(-1) of BW.d(-1), respectively) given in 2 equal meals to...

This study aimed to measure the effect of Catfish oil supplementation on rumen pH, volatile fatty acid (VFA), acetate: propionate, NH3, microbial protein, carboxymethyl cellulase (CMCase), and protease activity. There are five treatments conduct current i.e. T0 (control diet: Pennisetum purpupoides (60): wheat pollard (30): soybean meal (10)), T1 (T0 + 2% DM CFO), T2 4% T3 6% T4 8% CFO) each tr...

The activities of cellulolytic and xylanolytic enzymes produced by an anaerobic fungus (R1) which resembled Neocallimastix sp. were investigated. Carboxymethylcellulase (CMCase), cellobiase, and filter paper (FPase) activities had pH optima of 6.0, 5.5, and 6.0, respectively. CMCase and cellobiase activities both had a temperature optimum of 50 degrees C, whereas FPase had an optimum of 45 degr...

Cellulase and xylanase have been widely studied for bioconversion processes applied in various industries. The high cost of these enzymes remains to be the major bottleneck large-scale commercial application lignocellulosic biorefinery. use agroindustrial residues weeds as fermentation substrates is an important strategy increase cellulolytic production reduce costs. Penicillium crustosum was n...

Cellulase are important class of enzymes that has potential applications in textile, biofuel, food and feed industry. Fungi such as Aspergillus, Trichoderma reliable sources cellulase production. Optimisation multiple parameters is required for efficient production high titers both SmF SSF. Carbon lactose, maltose, cellulose, nitrogen including ammonium dihydrogen phosphate beef extract enhance...

The extracellular endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1 were analyzed by high-performance liquid chromatography (HPLC) by using DEAE ion-exchange, hydroxylapatite, and gel filtration chromatography and polyacrylamide gel electrophoresis (PAGE). Two endo-1,4-beta-glucanase peaks were resolved by DEAE-HPLC and termed endoglucanases A and B. Carboxymethyl cellulose (...

This work evaluates the enzymatic production of hydrolases and oxireductases by endophytic fungi isolated from leaves caesarweed (Urena lobata L.), which is a perennial plant that well adapted to Amazon floodplains region, being widely used in hessian sacks other products natural fiber. Fungi strains Fusarium sp. (1290) Corynespora (1291) were reactivated, had their DNA extracted sequenced obta...

Two mutant strains of Ruminococcus flavefaciens strain 007 that differ in their ability to hydrolyse cotton fibres have been shown also to differ in their cell-surface topology, in that the cotton degrading form possessed larger and more protuberant cell surface structures. The strains had similar CMCase, cellobiosidase and beta-glucosidase activities. The results indicate the importance of cel...

Five organic supplements namely cotton seed meal (CSM), peanut meal (PM), wheat bran (WB), rice bran (RB) and soybean meal (SBM) were supplemented to the wheat straw @ 10% and 20% (on dry weight basis). Mycelial growth and enzyme activity of Lentinus edodes strain Le-S were recorded at periodic intervals of 10 d upto 30 d. Maximum mycelial extension rate was obtained with 10% rice bran suppleme...