Nine isolates of cellulose-degrading Actinomycetes were isolated from different sediment samples from the Bhitarkanika Mangrove Forest. Cellulase and hydrolytic activity was confirmed with clear zone around the colony on basal salt agar medium using Congo Red. The enzyme assays for two enzymes, filter paper cellulase (FPCase), and carboxymethylcellulase (CMCase), were examined by methods recomm...

This study aimed to isolate and evaluate the cellulase activity of cellulolytic bacteria in hot springs of Dehloran, Ilam province, Iran. Water and sludge samples were collected from the hot springs and the bacterial enrichment was performed in a medium containing rice barn and carboxymethyl cellulose (CMC). The cultures were incubated at 50 °C in aerobic conditions. The bacteria were isolated ...

The aim of this work was to establish the optimal conditions for production of carboxymethylcellulase (CMCase) by a newly isolated marine bacterium using response surface methodology (RSM). A microorganism producing CMCase, isolated from seawater, was identified as Cellulophaga lytica based 16S rDNA sequencing and the neighborjoining method. The optimal conditions of rice bran, ammonium chlorid...

Validamycin A decreased the biomass and extracellular protein produced by cultures of Rhizoctonia solani A79 grown on medium containing cellulose as the carbon source. In the presence of the antibiotic, the production of inducible enzymes active on filter paper (FPase), carboxymethylcellulose (CMCase) and (cellobiase) was decreased, but not the production of constitutive enzymes involved in hem...

Objective: Peel of Amorphophallus is considered as waste. In this study we checked for the presence of cellulase enzyme in this waste and investigated its biochemical properties. Methods: Presence of cellulase activity was confirmed by Congo red plate diffusion assay The optimum temperature, pH, substrate concentration for both CMCase and FPase activity was also determined by DNS method. Effect...

Actinomycetes are Gram-positive filamentous bacteria well known for the production of bioactive compounds. Recently, many halophilic habitats have been explored isolation actinomycetes that exhibit biotechnological potentials. In this investigation, a saline habitat Rajasthan, Sambhar Salt Lake (SSL) was selected to study population and Carboxy Methyl Cellulase (CMCase) by native isolates. A to...

Carboxymethyl cellulase (CMCase) is the key enzyme used by bacteria to decompose the plant root hairwall during symbiosis. In this study, CMCase activities were routinely assayed using carboxymethyl cellulose (CMC) as a substrate. Coupled with the catalytic reaction of BCA (2,2’-bicinchoninic acid) solution, the color developed at 570 nm was measured to determine the enzyme activity. Sinorhizob...

The potential of untreated Parthenium hysterophorus weed biomass was evaluated as a substrate for cellulase production. The cellulose in the biomass was used as the main source of carbon. Solid-state fermentation was carried out using Trichoderma reesei, and optimization of cultural conditions was done for maximization of cellulase production. The results revealed that highest cellulase product...

Cellulase finds use in saccharification of lignocellulosic agroresidues to fermentable sugars which can be used for production of commercially important metabolites. This study reports endoglucanase (CMCase) production by Enhydrobacter sp. ACCA2. The CMCase activity of the strain ACCA2 was successively improved by optimization of range of physical and nutritional parameter in a set of non-stati...