ABSTRACT In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation of pronuclei, first cleavage zygotes, development preimplantation embryos even metabolism individuals after birth. The regulatory role sperm microRNAs (miRNAs) in bovine has not been reported detail. By constructing screening miRNA expression libraries, we f...

Uneven blastomere cleavage in human embryos of 'good morphology', i.e. those normally used for transfer, is a phenomenon which has been poorly investigated. The main objective in this study was to probe deeper into the aetiology behind previous findings that embryos with uneven cell cleavage have a lower developmental capacity in comparison with evenly cleaved embryos. Our hypothesis was that u...

This study was conducted to study the kinetics of initial cell divisions in relation with the cleavage patterns in viable (with the ability to develop to the blastocyst stage) and non-viable bovine embryos and parthenotes. The kinetics of in vitro development and cleavage patterns were observed by time lapse cinematography. The length of the first and second but not third cell cycle differed si...

BACKGROUND The need for effective parameters for selecting the best embryos is paramount when a large number of them are available for transfer. Other studies have reported that transfer of pre-selected embryos, based on cleavage to the 2-cell stage at 25 h and 27 h post-insemination/intracytoplasmic sperm injection (ICSI), increases implantation and pregnancy rates. We investigated whether ext...

INTRODUCTION HLA-G is a major histocompatibility complex (MHC), class Ib molecule that is selectively expressed at the fetal-maternal interface. It is thought to play a role in protecting the fetus from the maternal immune response. Interestingly, the preimplantation embryo development (Ped) gene product Qa-2 is also a mouse MHC class Ib protein that affects cleavage and division of preimplanta...

Human embryos for hESC derivation are often donated at the cleavage stage and of reduced quality. Poor quality embryos have lower efficiency for hESC derivation. However, cleavage stage mouse embryos develop into higher quality expanded blastocysts if they are cultured with insulin, suggesting that this approach could be used to improve hESC derivation from poor quality cleavage stage embryos. ...

background: timing of the first zygotic cleavage is an accurate predictor of embryo quality. embryos that cleaved early (ec) have been shown to exhibit higher developmental viability compared to those that cleaved at a later period (lc). however, the viability of ec embryos in comparison to lc embryos after vitrification is unknown. the present study aims to investigate the post-vitrification d...

AIM The aim of this study was to investigate the relationship between the morphology, euploidy and implantation rate of cleavage stage and blastocyst stage embryos. SETTING Institution-based, tertiary care in-vitro fertilization centre. STUDY DESIGN This study included a retrospective data analysis of 306 embryos: 154 cleavage stage embryos and 152 blastocysts that underwent biopsy on day 3...

Background: Research studies on reproductive mechanism of laboratory animals are essential for further advancement of assisted reproductive techniques (ART). One of these studies includes the assessment of in-vitro development of pre-implantation embryos. The objective was to compare the cleavage rates and morphology of in-vivo formed 2 to 8 cell embryos and blastocysts with in-vitro culture of...

This study aimed to evaluate the effect of essential (EAAs) and non-essential (NEAAs) amino acids (AAs) at levels of 25 and 25 or 50 and 50 μl/ml, respectively, on in vitro developmental potential of rabbit embryos at 4and 8-cell stages. Total of 12 sexual mature New Zealand White (NZW) rabbit does and 3 fertile NZW bucks were used in this study. All does and bucks were kept under similar condi...