On-plate enrichment of phosphopeptide digests followed by MALDI-MS is attractive for analyzing small quantities of phosphoproteins because it involves minimal sample handling and reduces sample loss. This work describes a method for modification of Si wafers, which serve as MALDI plates, with 250 microm-diameter microspots of phosphopeptide-binding polymer brushes enclosed by a hydrophobic poly...

The effects of dietary protein on Ca bioavailability and utilization in bone were examined in male spontaneously hypertensive rats (SHR) fed on diets containing either casein (200 g/kg (control), 60 g/kg or heat-damaged (HD) 200 g/kg) or soya-bean protein isolate (200 g/kg (control), 60 g/kg, or HD 200 g/kg). Casein was heat-damaged to limit casein-phosphopeptide (CPP) production in order to ev...

background: this study was done to evaluate remineralizing potential of bioactive glasses (bags)and amorphous calcium phosphate‑casein phosphopeptide (acp‑cpp) on early enamel lesion.materials and methods: twenty freshly extracted mandibular premolars were sectioned sagittally.the buccal half was impregnated in acrylic resin blocks and treated with 37% phosphoric acid inliquid form, to deminera...

Background: The cause of cavities is initially due to roughness on the tooth surface, requiring fluoride varnish prevent caries, as applies a compound surface. Fluoride reacts with enamel surface form calcium and fluorapatite, thus making more resistant demineralization damage. Purpose: This study aims compare surfaces among three varnishes under acidic conditions. Methods: research method uses...

OBJECTIVES This study aimed to assess the effect of applying casein phosphopeptide- amorphous calcium phosphate (CPP-ACP) paste, casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) paste and sodium fluoride gel on surface microhardness of enamel after microabrasion. MATERIALS AND METHODS Thirty freshly extracted human premolars were selected. All samples were subjected to ha...

Objective: One complication of fixed orthodontic treatment in patients with poor oral hygiene is development of demineralized enamel lesions compromising esthetics. This study aimed to review preventive measures and treatment of white spot lesions (WSLs) in patients with fixed orthodontic appliances. Review of Literature: In this review study, PubMed and Google Scholar databases were searched ...