effect of controlled atmospheres (cas) at various concentrations of co2, n2 and o2 on the lethal times of tribolium castaneum and trogoderma granarium was investigated at 20 and 30 ºc. experiments were performed using a recirculatory multi-flask apparatus. the results revealed that, the shortest times (0.1, 0.3 and 0.9 day for adults, larvae and pupae, respectively) required to obtain 50% morta...

IMPORTANCE OF THE FIELD The functional contribution of membrane-bound extracellular carbonic anhydrases (CAs) to hypoxic tumor growth and progression has long been hypothesized; however, recent convergent evidence from a number of groups strongly implicates these CAs as key prosurvival enzymes during tumor hypoxia. From this perspective targeting the inhibition of cancer-associated CA enzymes, ...

Bacterial adaptive immunity utilizes RNA-guided surveillance complexes comprising Cas proteins together with CRISPR RNAs (crRNAs) to target foreign nucleic acids for destruction. Cas9, a type II CRISPR-Cas effector complex, can be programed with a single-guide RNA that base pairs with the target strand of dsDNA, displacing the non-target strand to create an R-loop, where the HNH and the RuvC nu...

The extracellular matrix exerts a stringent control on the proliferation of normal cells, suggesting the existence of a mitogenic signaling pathway activated by integrins, but not significantly by growth factor receptors. Herein, we provide evidence that integrins cause a significant and protracted activation of Jun NH2-terminal kinase (JNK), while several growth factors cause more modest or no...

Objective: The purpose of the work was to investigate new synthetic compounds imidazolinone-based sulfonamide derivatives as potent and selective enyzme inhibitors. A number synthesized their inhibitory action against acetylcholine esterase (AChE), human (h) carbonic anhydrase (CA) isoforms I II were investigated. Material Method: identity has been confirmed by HRMS, 1H NMR, 13C NMR. pharmacolo...

Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be dire...

The alpha carbonic anhydrases (α-CAs) are a group of structurally related zinc metalloenzymes that catalyze the reversible hydration of CO2 to HCO3(-). Humans have 15 different α-CAs with numerous physiological roles and expression patterns. Of these, 12 are catalytically active, and abnormal expression and activities are linked with various diseases, including glaucoma and cancer. Hence there ...

The extracellular matrix exerts a stringent control on the proliferation of normal cells, suggesting the existence of a mitogenic signaling pathway activated by integrins, but not significantly by growth factor receptors. Herein, we provide evidence that integrins cause a significant and protracted activation of Jun NH 2 -terminal kinase (JNK), while several growth factors cause more modest or ...