The imaged capillary isoelectric focusing method was developed and validated for charge variant analysis of the bevacizumab innovator product its biosimilar formulations (N = 2). optimized consists of; 4 M urea, 2.0 mM iminodiacetic acid, 0.35% m/v methylcellulose, 5.0 L-arginine, 4.0% v/v carrier ampholytes (narrow-range point 5–8 8–10.5 with ratio 1:3), 60 sodium hydroxide 30 phosphoric acid ...

We developed a simple and highly-sensitive enzyme activity assay method based on reagent release capillary – isoelectric focusing (RRC-IEF), that can be applied for the development of multi analyte sensing device allowing simple and highly sensitive multi assay of both proteins and enzyme activities.

We report a multiplexed capillary electrophoresis system employing an array of 32 capillaries with a micromachined sheath-flow cuvette as the detection chamber. The sample streams were simultaneously excited with a 473-nm laser beam, and the fluorescence emission was imaged on a CCD camera with a pair of doublet achromat lens. The instrument produced mass detection limits of 380 +/- 120 yoctomo...

DOI: 10.1002/elps.202300039 This cover image shows a highly sensitive imaged capillary isoelectric focusing (icIEF) immunoassay for studying adeno-associated virus (AAV) capsid protein charge heterogeneity. AAVs are denatured and loaded into the of Peggy Sue™ instrument. Separated proteins immobilized to wall immunodetection using antibodies targeting three (VP1, VP2, VP3). method combines reso...

A novel acid proteinase (Tropiase) was isolated from Candida tropicalis IFO 0589 by DE52-cellulose, and DEAE-Cosmogel column chromatographies. The purified tropiase gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme preparation had a molecular weight of 23,900, isoelectric point of p...