Echium amoenum Fisch. & Mey. (Boraginaceae) is a very popular medicinal plant that is used as a tonic, tranquillizer, diaphoretic, and a remedy for cough, sore throat and pneumonia in traditional medicine of Iran. Callus culture of medicinal plants is one of the ways for production of secondary metabolites. In this study, we investigated callus culture of E. amoenum and the major secondary meta...

Echium amoenum Fisch. & Mey. (Boraginaceae) is a very popular medicinal plant that is used as a tonic, tranquillizer, diaphoretic, and a remedy for cough, sore throat and pneumonia in traditional medicine of Iran. Callus culture of medicinal plants is one of the ways for production of secondary metabolites. In this study, we investigated callus culture of E. amoenum and the major secondary meta...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

Agrobacteria mediated Coleus blumei tumour tissues were cultured in vitro on MS medium. Sixteen diversified transformed callus cultures were maintained for several years in the absence of plant growth regulators and antibiotics without affecting the growth rate. Rosmarinic acid was detected spectrophotometrically in all tissue lines but in different quantities. The highest rosmarinic acid accum...

Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropa­ noids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was com­ pared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoi...

background: sugarcane is a monocotyledonous crop that is cultivated in the tropical and subtropical regions of the world. one of the most important criteria, influencing the efficiency of the sugarcane transformation is known to be related to physical and biological factors during the transformation procedure. objectives: the objective of this research was to study the response of callus induc...

Feasible and efficient tissue culture plays an important role in plant genetic engineering. Wheat (Triticum aestivum L.) immature embryos (IMEs) are preferred for tissue culture to mature embryos (MEs) because IMEs easily generate embryogenic callus, producing large number of plants. The molecular mechanisms of regulation and the biological pathways involved in embryogenic callus formation in w...