In this study, n-butanol fraction (KBF) and aqueous fraction (KAF) isolated from methanol extract of Koelreuteria paniculata Laxm. leaves were evaluated for their antioxidant potential using (ABTS) 2, 2-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-2-picrylhydrazyl) assay, Reducing power assay and Superoxide anion radical scavenging assay. These fractions were also studi...

The interaction of native calf thymus DNA (CTDNA) with azorubine in 10 mM Tris–HCl aqueous solution at neutral pH 7.4 was monitored by UV-vis absorption and voltammetric techniques. Upon addition of the azorubine, UV absorption band of DNA decreased. After addition different concentrations of CT-DNA, UV absorption band of azorubine showed hyperchromisity. The binding constants (Kb) for the azor...

The mechanism by which millimolar concentrations of ATP stimulate the activity and increase the processivity of calf thymus DNA polymerase alpha has been investigated with poly(dA)/oligo(dT) as template/primer to eliminate possible effects due to primer synthesis. The effect of ATP on the rate of DNA synthesis with this template/primer was found to be dependent upon whether or not the ATP was n...

This work reports that the rheology of ideally monodisperse lambda ( λ $$ \lambda ) phage DNA solutions is extremely sensitive to applied strain in dynamic oscillatory experiments. exhibits nonlinearity at strains far smaller than what observed conventional synthetic polymers. However, it found polydisperse calf thymus does not exhibit extreme sensitivity. By mixing samples with DNA, we correla...

Complement-fixing anti-DNA antibodies were detected in a minority of sera of rabbits hyperimmunized with killed Gram-negative bacteria. The C'-fixing property of DNA was lost after DNase treatment. Preferential reactivity with denatured DNA was observed. The antisera reacted with DNA preparations derived from rabbit bone marrow and thymus, calf thymus, pneumococci, salmon sperm, and Escherichia...