A B S T R C TELISA (Enzyme-linked immunosorbent assay) is a technique used to assessthe quantification of peptide, protein, antibody and hormone levels, basedon the principle antigen-antibody binding. In ELISA technique, antigenimmobilization will be carried out on solid surface, then bound withantibodies form an bond complex, where complex enzyme. The detection signal in formof color change fo...

Magnetic nanoparticles (MNPs) have emerged as a promising material in disease diagnostics due to their potential enhance detection sensitivity, facilitate concentration and purification of target substances diverse samples, enable favorable color-based detection. In this study, antibody-conjugated MNPs were successfully synthesized validated through two appropriate methods: the measurement MNPs...

      A highly sensitive electrochemicalimmunosensor was developed to detect hCG based on immobilization ofhCG-antibody (anti-hCG) onto robust nanocomposite containing Gr, Chit,1-methyl-3-octyl imidazolium tetra fluoro borate ionic liquid (IL)(Gr-IL-Chit). AuNPs were used to immobilize hCG antibody on the modifiedelectrode. The amine groups of the antibody are cova...

Background and aims: Toxoplasmosis is a parasitic disease which may cause some laboratory symptoms in infected individuals. One of the main ways of transmition this organism is placenta to fetus pathway. If this transmission occurs in the 3th month of pregnancy, the abortion, central nerve system and ocular disorder will happen. Because of this issue, the precise technique for detection of Toxo...

p53 is a key tumor suppressor gene that is targeted for inactivation during human tumorigenesis. In this study, we produced and characterized polyclonal antihuman p53 antibody. The cDNA encoding the completehuman p53 protein was cloned into pGEX-4T-1 and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase (GST). The rabbits were immunized...

Borreliacidal antibody has been shown to be important for the serodiagnosis of Lyme disease and determination of immune status. Our results show that borreliacidal antibody can be rapidly and accurately detected by flow cytometry. Acridine orange was added to normal and immune sera containing Borrelia burgdorferi organisms in the presence and absence of complement prior to data acquisition by f...

  Antibody detection is the most versatile and commonly used method for measles diagnosis. Detection of specific IgM antibodies in a single serum specimen collected within the appropriate time after rash onset can provide a good presumptive diagnosis of current or recent measles infection and is the test of choice for rapid diagnosis of measles cases. So, optimal timing for collection of a sing...