abstract the addition of manures and chemical fertilizers as soil amendments for the improvement of soil fertility and quality could affect the seasonal changes in the activity of soil microbes and enzymes. this study aimed at investigating the effect of different levels of nitrogen from broiler litter and urea sources on the trend of urease, alkaline phosphatase and saccharase activities in a ...

objective the aim of this study was to determine the effect of revival soymilk consumption and moderate exercise on vasomotor symptoms, total antioxidant capacity (tac) and alkaline phosphates activity in postmenopausal women. materials and methods in this study, daily for 3 months, 37 postmenopausal women (mean age = 52.2± 4.6 years) were randomly assigned to soymilk (n=15), exercise +soymilk ...

the effects of dietary vitamin e levels on mucosal maltase and alkaline phosphatase (alp) enzyme activities and on the amount of mucosal malonyldialdehyde (mda) in broiler chickens were studied in the present study. one hundred and eighty of day old male broiler chicks (ross 308 strain) were randomly assigned into five groups, each with three replicates and 12 chicks in each replicate. chickens...

The specificity of the enzyme-linked immunosorbent assay(s) is thought to depend on the specificity of the antibody used in the assay system. Therefore, the association of broadly reactive antigens like endotoxin with enzyme conjugates or other enzyme-linked immunosorbent assay reagents has the potential of altering the specificity of reactions in the enzyme-linked immunosorbent assay. Using th...

The aim of the research was to identify promising biochemical markers blood associated with intensity live weight gain in young cattle. work carried out 2021...2022 conditions dairy farm LLC Severnaya Niva Kortkerossky district Komi Republic. A group newborn heifers Kholmogorsky breed (n = 30) selected for experiment. It has been established that on first day after birth, calves intensive growt...

Three FLAG epitopes have been incorporated into the mammalian expression vector pCMV-5 to create a transient expression vector, p3XFLAG-CMV-7. The vector was designed to express FLAG fusion proteins that can be detected at tenfold lower expression levels than the current FLAG fusion protein expression system. The usefulness of this expression and detection system was demonstrated by expression ...

A solid phase antibody assay by means of alkaline phosphatase conjugated to antiimmunoglobulin is described. Specially designed microcuvettes were sensitized with influenza A antigen, and antibodies bound to it were assayed by anti-IgG alkaline phosphatase conjugate in a semiautomated photometer equipped with a programmable calculator. The sensitivity was found to be 200 times higher than HI- o...

Bone alkaline phosphatase was evaluated by wheat-germ lectin precipitation in several clinical conditions. The study included 33 premenopausal healthy women, 46 postmenopausal apparently healthy women, 19 growing children, 24 patients with Paget's disease, 31 patients with primary hyperparathyroidism and 66 patients with hepatobiliary diseases. In postmenopausal women the mean T score (i.e.: th...