aflatoxin contamination of food and feedstuffs is a serious economic and health hazard. over a period of two years, 35 samples of raw pistachio nuts collected from different parts of iran were analysed for the presence of aflatoxigenic strains of the aspergillus flavus group. eighty-five isolates of a.flavus and a.parasiticuswere identified based on specific yello-orange pigment production in t...

backgrounds and objectives: to obtain information about aflatoxigenicity of isolated aspergillus flavus strains from shrimps. material and methods: forty - three isolates of aspergillus flavus from cultured green tiger shrimps of persian gulf were examined for their ability to produce aflatoxins. initially two media; aflatoxin producing ability medium and coconut agar medium were used to detect...

Liquid chromatography - high resolution mass spectrometry (LC-HRMS) developed to screen some residues in rice. Pesticides, aflatoxin B1, B2, G1, aflatoxin G2 and quaternary ammonium surfactants were detected by the optimized method. Rice samples extracted cleaned QuEChERS then analyzed QExactive system with HESI ionization source for both negative positive modes. The&...

background: aflatoxins are toxic fungal metabolites enable to contaminate a wide range of natural substrates. this contamination can be host-specific for different plant species. in this study, the ability of a toxigenic aspergillus parasiticus to produce various aflatoxins on major iranian cereals was evaluated with special focus on plant susceptibility to toxin production at cultivar level. m...

Aflatoxins are carcinogens produced by Aspergillus flavus, A. parasiticus and A. nomius. In the present study, peanut samples were collected at different phenological stages of the plant during the 2007/2008 and 2008/2009 seasons and from stored peanuts harvested in 2007/2008. The mycoflora and aflatoxins in the peanuts were evaluated. The results showed the presence of Fusarium spp., Macrophom...

Antibody based techniques are widely used for the detection of aflatoxins which are potent toxins with a high rate of occurrence in many crops. We developed a murine monoclonal antibody of immunoglobulin A (IgA) isotype with a strong binding affinity to aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) and aflatoxin M1 (AFM1). The antibody was effectively used i...

Stored and cooked samples of pearl millet (Pennesetum typhoides), which is regularly consumed as food by the Paharia tribe in the hilly regions of Santhal Pargana, Bihar State, India, that were harvested in January 1989 were analyzed for mold flora, natural occurrence of Aspergillus flavus and A. parasiticus, and incidence and levels of aflatoxin B1. Of the 22 fungal species isolated, A. flavus...

Aflatoxin B1 poses grave threats to food and feed safety due to its strong carcinogenesis and toxicity, thus requiring ultrasensitive rapid on-site determination. Herein, a portable immunosensor based on chromatographic time-resolved fluoroimmunoassay was developed for sensitive and on-site determination of aflatoxin B1 in food and feed samples. Chromatographic time-resolved fluoroimmunoassay o...