The establishment of an efficient Agrobacterium–mediated transformation protocol for a recalcitrant crop like high oleic sunflower genotypes, cv. Capella and SWSR2 inbred line, is presented in this paper. The protocol requires the identification and optimization of parameters affecting T-DNA delivery and plant regeneration. The basis for identifying the most appropriate conditions for transform...

Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest l...

Sorghum serves as staple food for millions of people in Asia. It ranks as the sixth most planted crop and it is vulnerable to fungal diseases resulting in decreased grain quality and yield loss. Agrobacterium strains used were LBA4404 harbouring pcambia-ubi-chi11 (rice chitinase), EHA105 harbouring pcambia-ubi RC7 (rice chitinase) with bar gene and EHA105 harbouring pMKURF2 (rice chitinase gene...

An efficient variety-independent method for producing transgenic eggplants (Solanum melongena L.) via Agrobacterium tumefaciens-mediated genetic transformation was developed. Two weeks old cotyledon explants were transformed by co-cultivation with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pVDH65 carrying the reporter gene β-glucuronidase intron (GUS-INT) and the marker ...

Valsa mali is a causal agent of apple and pear trees canker disease, which is a destructive disease that causes serious economic losses in eastern Asia, especially in China. The lack of an efficient transformation system for Valsa mali retards its investigation, which poses difficulties to control the disease. In this research, a transformation system for this pathogen was established for the f...