A 50 year old gentleman presented to our surgical outpatient department with two years history of bleeding per rectum with off and on lower abdominal pain. He was pale, lethargic and emaciated. On digital rectal examination, hard fungating cauliflower like growth was found at anorectal junction. Systemic examination revealed no significant findings. Laboratory investigations showed Hb l0gm/dI. ...

We have demonstrated previously that interferon (IFN)sensitizes human colon carcinoma cell lines to the cytotoxic effects of 5-fluorouracil combined with leucovorin and to the thymidylate synthase inhibitor, ZD9331, dependent on thymineless stress-induced DNA damage, independent of p53. Here we demonstrate that the cyclin-dependent kinase (CDK) inhibitor p21 regulates thymineless stress-induced...

The predictive utility of several biochemical parameters of 5fluorouracil (5-FUra) action was evaluated in four murine coIonic adenocarcinomas: 5-FUra-sensitive Tumor 38 and 5FUra-resistant Tumors 07/A, 51, and 06/A. Thymidylate synthetase (TS) was determined by a tritiated 5-fluoro-2'-deoxyuridylate (FdUMP)-binding assay. Bolus 5-FUra (80 mg/kg, i.p.) administration caused in all tumors a rapi...

The purpose of these studies was to determine the possible mechanisms responsible for the therapeutic effects of systemic administration of 5fluorouracil (FUra) and 7-interferon on disseminated human colon cancer. We used several human carcinoma cell lines that were established from different surgical specimens. Some lines were selected in nude mice for increased metastatic potential, and one l...

phagia secondary to squamous cell carcinoma of the esophagus, staged as T4N1M0. A covered self-expandable metal stent (SEMS) (Choostent; Solco Intermed, Seoul, Korea) was inserted for symptomatic relief, chemoradiotherapy was performed with carboplatin and 5fluorouracil, and this was followed by radiotherapy to deliver 39.6 Gy in 22 daily fractions. The patient presented with cough, thoracic pa...

Because of the association between the incorporation of 5fluorouracil (FUra) into RNA and cytotoxicity, uridine was ex amined for potential selective reduction of host toxicity. Male BALB/c x DBA/2 F, mice (tumor free or bearing advanced colon tumor 26) were used. Two uridine schedules (each beginning 2 hr after FUra) have been successful: (a) uridine at 800 mg/kg every 2 hr for three doses fol...

Purpose: The aim of this study was to investigate the antitumor effect of the chloroform and ethanol extract of the whole plant of Cuscuta reflexa Roxb. (Cuscutaceae) in Swiss albino mice against Ehrlich Ascites Carcinoma (EAC) cell line. Methods: The antitumor activity of the chloroform and ethanol extracts of Cuscuta reflexa was evaluated against Ehrlich ascites carcinoma (EAC) tumor in mice ...

Dihydropyrimidine dehydrogenase is the most extensively investigated predictive marker for individual response to 5fluorouracil. Clinical responses to the anticancer agent, along with various reports, have clearly shown that dihydropyrimidine dehydrogenase activity is closely correlated to its mRNA levels, but the regulatory mechanisms of its expression have remained unclear. We attempted to cl...

Purpose: To design and develop halogenated chalcone derivatives and evaluate them as anticancer agents using different cancer cell lines. Methods: Based on in silico design and docking on known target, crystal structure of the complex of interleukin-1beta converting enzyme (ICE) with a peptide based inhibitor, (3S )-N-Methanesulfonyl-3({1-[N-(2-naphtoyl)-l-valyl]-l-prolyl}amino)-4-oxobutanamide...

Purpose: STAT3 is known to have both oncogenic and tumor suppressive effects, but the regulation of these opposing effects is elusive. We hypothesized that STAT3b, one of the two STAT3 isoforms, is the key determinant in this context. Experimental Design: The prognostic significance of STAT3b and phospho-STAT3a (pSTAT3a) was evaluated in 286 cases of patients with esophageal squamous cell carci...