Enteroaggregative Escherichia coli (EAEC) adherence to human intestinal tissue is known to be mediated by aggregative adherence fimbriae (AAF). However, the host cell molecules involved in EAEC adherence remain uncharacterized. In the present study, four key membrane glycoproteins of cultured human intestinal epithelial cells (INT-407) were found to be involved in the interaction with the T8 st...

Monolayers of intestine 407 (Int-407) cells were infected with the virulent Salmonella typhimurium strain C52, and the adhesion to and invasion of these cells were studied. The effects of the multiplicity of infection and growth phase of the bacteria (logarithmic versus stationary) on the interaction with eukaryotic cells were investigated. In contrast to other reports, we found no differences ...

the objective of this work was to develop bioadhesive topical gel of aceclofenac with the help of response-surface approach. experiments were performed according to a 3-level factorial design to evaluate the effects of two independent variables [amount of poloxamer 407 (pl-407 = x1) and hydroxypropylmethyl cellulose k100 m (hpmc = x2)] on the bioadhesive character of gel, rheological property o...

Antibodies to neuropeptide receptors can be used to localize and characterize the receptors in tissues and cell lines. Two strategies were used to study the rat substance P receptor (SPR, NK-1) by immunological methods. First, a polyclonal antiserum was raised by immunizing rabbits with a peptide corresponding to the 15 amino acid residues (KTMTESSSFYSNMLA, SPR393-407) at the intracellular C-te...

OBJECTIVE To compare the acute toxicity of common injection and sustained-release preparation of norcantharidin for mice so as to identify the attenuation of the sustained-release preparation of norcantharidin. METHODS The poloxamer 407 was used as a sustained-release vehicle for topical administration of norcantharidin, and the acute toxicity of mice treated with common injection and sustain...

BACKGROUND Estimates of health problems of the elderly in developing countries are required from time to time to predict trends in disease burden and plan health care for the elderly. Developing countries have a poor track record of equitable distribution of health care. Marginalized groups living in urban slums and rural villages have poor penetration of health services. AIMS To identify the...

Classically, a computation operates on a string of bits, applying a sequence of physically realizable operations (such as logical NOT, AND, and OR), and outputs an answer based on the value of the last obtained bit string. Similarly, a quantum computation operates on a register consisting of a number of quantum bits (qubits), applying a sequence of (hypothetically) physically realizable quantum...

Do we have non-relativizing techniques? Yes, we do! In fact, already the proof of the CookLevin theorem is non-black-box: to reduce the computation of a nondeterministic TM on a given input x to a 3-cnf φx, we had to “open up” the TM and look inside at the sequence of configurations the TM goes through, and moreover, exploit a very special property of a TM computation: that the computation is l...

Crystals of intact small ribosomal subunits from Thermus thermophilus have been obtained from functionally active particles. The crystals (P42(1)2, 407 A x 407 A x 171 A) are suitable for X-ray crystallography analysis to 9.9 A using synchrotron radiation at cryotemperature. Crystallographic data from native and a potential heavy-atom derivative have been collected.

s 2008;112:407.