We investigated the effect of arginine vasopressin (AVP) on inorganic phosphate (Pi) transport in A-10 rat aortic vascular smooth muscle cells (VSMCs). AVP time- and dose-dependently stimulated Na-dependent Pi transport in A-10 cells. This stimulatory effect of AVP on Pi transport was markedly suppressed by V1 receptor antagonist. A protein kinase C (PKC) inhibitor calphostin C partially suppre...

Previously, we have demonstrated that 17α-ethynylestradiol (EE) induces rat multidrug-resistance associated protein 3 (Mrp3, Abcc3) expression transcriptionally through estrogen receptor-α (ER-α) activation. We explored the effect of EE on MRP3 expression of human origin. HepG2 cells were transfected with ER-α and incubated with EE (1-10-50 μM) for 48 h. MRP3 protein and mRNA levels were measur...

BACKGROUND The overall objective of this study was to investigate neuronal apoptosis and expression of apoptosis related proteins (c-jun, cytc and Bax) in the cerebellum of rates with heroin addiction. MATERIAL/METHODS 40 adult male Sprague-Dawley rats which weighing 200-220 g were randomly divided into 5 groups (n = 8 per group): control group, 10-day heroin-addicted group, 20-day heroin-add...

The effect of mechanical strain on transcription and expression of the immediate-early genes, early growth response gene-1 (Egr-1), c-jun, and c-fos, was investigated in neonatal rat aortic vascular smooth muscle (VSM) cells. Cells grown on silicone elastomer plates were subjected to cyclic mechanical strain (1 Hz) at various durations and magnitudes. Egr-1 mRNA increased rapidly in response to...

10-Formyltetrahydrofolate dehydrogenase (FDH) suppresses cancer cell proliferation through p53-dependent apoptosis but also induces strong cytotoxicity in p53-deficient prostate cells. In the present study, we have shown that FDH induces apoptosis in PC-3 prostate cells through simultaneous activation of the c-Jun-NH(2)-kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways with ...

To analyze the function of AP-1 in cartilage formation, two types of primary chondrocytes, LS and US cells, were prepared from caudal (lower) and cephalic (upper) regions of chicken sterna, respectively. All the known components of chicken AP-1 (c-Fos, Fra-2, c-Jun, and JunD) were detected in both cell types, but the expression level of c-Jun was much higher in LS cells, which are rich in less ...

The cardiac myocyte is a terminally differentiated cell. In response to increased myocardial workload or damage, myocytes undergo hypertrophic (as opposed to hyperplastic) growth. This response includes transient upregulation of immediate early genes such as c-jun [ 1,2]. Expression of c-jun is partly controlled by cJun/ATF2 dimers bound to AP-1-like sites in the c-jun promoter [3,4]. Phosphory...

We compared the ability of cellular and viral Jun (c-Jun and v-Jun) to transactivate target genes. c-Jun and v-Jun bind specifically to 12-O-tetradecanoylphorbol-13-acetate responsive elements [TREs, also called activator protein 1 (AP-1) motifs]. However, whereas c-Jun activates TRE-controlled promoters, v-Jun represses them. Cotransfection of the two Jun proteins reduces c-Jun-dependent trans...

Immediate-early genes (IEGs) are transiently expressed within the rodent circadian system in response to nocturnal light. The two most studied light-induced IEGs within this system are Fos and Jun-B. Molecular expression of these two genes within the hypothalamic suprachiasmatic nucleus (SCN) correlates with light-induced behavioral phase shifts. Previous studies of the role of Fos and Jun-B in...

"Volume 2022 (2022): Issue 151 (Jun 2022): Cyprus: Article IV Consultation-Press Release; Staff Report; and Statement by the Executive Director for Cyprus" published on 01 Jun International Monetary Fund.