Background: Multidrug-resistant Acinetobacter baumannii is associated with life threatening nosocomial infections and hospital outbreaks. The organism is resistant to most commonly used antibiotics including carbapenems. Aim and Objectives: To study carbapenemase production among multidrug-resistant clinical isolates of A. baumannii. Material and Methods: Fifty multidrug-resistant A. baumannii ...

We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.

The fight against infectious diseases is probably one of the greatest public health challenges faced by our society, especially with the emergence of carbapenem-resistant gram-negatives that are in some cases pan-drug resistant. Currently,β-lactamase-mediated resistance does not spare even the newest and most powerful β-lactams (carbapenems), whose activity is challenged by carbapenemases. The ...

BACKGROUND & OBJECTIVE The production of carbapenemases is an important mechanism responsible for the carbapenem resistance. A simple and inexpensive testing method for screening of carbapenemase producers is essential. A prospective study was undertaken to detect metallo-beta-lactamases (MBLs) and AmpC beta-lactamases in nonfermentative Gram negative bacteria and to evaluate the various method...

BACKGROUND Emergence of carbapenems-resistant K. pneumoniae represents a serious challenge for antimicrobial therapy. OBJECTIVE The aim of this research is to determine different mechanisms mediating the emergence of K. pneumoniae isolates with high-level carbapenem resistance. METHOD A total of 80 K. pneumoniae isolates were purified from sputum and urine specimens. The minimum inhibitory ...

A flow cytometry test was developed to identify carbapenemase production by Enterobacteriaceae and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. It represents a convenient, fast, and reliable approach (1...

objectives the objective of this study was to develop the primers for single and/or multiplex pcr amplification assays for simultaneous identification of class a, class b, and class d carbapenem hydrolyzing β-lactamases in enterobacteriaceae and then to evaluate their efficiency. conclusions we have reported here a reliable method, composed of single and multiplex pcr assays, for screening all ...