We have analyzed the distribution and prognostic significance of terminal deoxynucleotidyl transferase (TdT) and adenosine deaminase (ADA) in connection with conventional cytology, cytogenetics, response to therapy, and survival. The study population consisted of 78 patients with AML, 44 patients with Ph1 + CML in chronic phase, and 35 adult patients with Ph1 + CML in blastic phase, among which...

Terminal deoxynucleotidyl transferase (TdT) was determined by immunofluorescence in 30 patients with leukemia. In acute lymphocytic leukemia the proportion of cells positive for TdT was 19 to 77 percent during relapse (12 cases) and less than one percent during remission (three cases). In seven cases of myeloproliferative disease and two cases of lymphoma, the TdT was less than one percent. In ...

The National Institute of Standards and Technology (NIST) administered the sixth open evaluation of Topic Detection and Tracking (TDT) technologies in November of 2003. The TDT project supports development of technologies that automatically organize eventrelated news stories. The program leverages expertise in core technologies, Automatic Speech Recognition (ASR), Document Retrieval (DR), and M...

Administration of dexamethasone to rats lyses greater than 75% of bone marrow and thymic lymphocytes. including those containing terminal deoxynucleotidyl transferase (TdT). Reappearance of TdT containing lymphocytes in bone marrow appears to be a phase of synchronized regeneration in which a cohort of cells differentiates together over a period of 2 wk. Days 1 -5 of marrow regeneration are cha...

An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL(-1), and the limit of detection for TdT was 0.25 U mL(-1).

Cases of acute leukemia showing both terminal deoxynucleotidyl transferase (TdT) and myeloperoxidase (MPO) activities are usually classified as acute myelogenous leukemia (AMI). Although some reports have implied overlap of TdT and MPO based on population percentages. direct evidence for simultaneous expression of TdT and MPO by a leukemic blast is lacking. By use of a simple new technique deve...

The National Institute of Standards and Technology (NIST) administered the second open evaluation of Topic Detection and Tracking (TDT) technologies in 1999. The TDT project supports development of technologies that automatically organize event-related news stories. The program leverages expertise in core technologies, Automatic Speech Recognition (ASR), Document Retrieval (DR), and Machine Tra...

A new form of TdT mRNA has been identified by screening a mouse thymus cDNA library. It contains an open reading frame of 1527 base pairs corresponding to a protein containing 509 aminoacids, whereas the previously identified mouse TdT mRNA is composed of 1587 base pairs and encodes a protein of 529 aminoacids. Analysis of a mouse genomic clone containing the 3' portion of the TdT gene shows th...

Terminal deoxynucleotidyl transferase (TdT) is an intracellular protein characteristic of certain primitive lymphocytes in normal thymus and bone marrow. It can be detected by enzyme assay or immunocytochemical staining. High levels of enzyme and increased numbers of TdT+ cells are found in some lymphoblastic leukemias and lymphomas. Analysis for TdT thus provides a useful adjunct in the differ...

Terminal deoxynucleotidyltransferase (TdT), which template-independently synthesizes DNA during V(D)J recombination in lymphoid cells, is ubiquitylated by a BPOZ-2/Cul3 complex, as the ubiquitin ligase, and then degraded by the 26 S proteasome. We show here that TdT is ubiquitylated by the Cul3-based ubiquitylation system in vitro. Because TdT could also be ubiquitylated in the absence of Cul/B...