نتایج جستجو برای: surface display
تعداد نتایج: 784206 فیلتر نتایج به سال:
Surface display is a powerful technique that uses natural microbial functional components to express proteins or peptides on the cell exterior. Since the reporting of the first surface-display system in the mid-1980s, a variety of new systems have been reported for yeast, Gram-positive and Gram-negative bacteria. Non-conventional display methods are emerging, eliminating the generation of genet...
We describe an efficient approach to rendering a perspectively correct image on a potentially irregular display surface that may be illuminated with one or more distinct devices. The first pass of the technique generates an image of the desired graphics model using conventional rendering. The second pass projects that image as a texture onto a model of the display surface, then re-renders the t...
Front-projection display systems suffer from a fundamental problem: the user can easily and inadvertently block the projector, creating shadows. The framework we present in this paper enables a front-projected display system to solve this problem and remove display surface shadows in real-time. Shadows are an example of radiometric inconsistencies, which cause a number of challenges in building...
In this study, the TiO2 nanorods were synthesized from P25 TiO2 nanoparticles by hydrothermal method in 10 M NaOH solution. The effects of annealing temperatures on produced nanorods were investigated by scanning electron microscopy (SEM) X-ray diffraction (XRD) and photoluminescence (PL) spectroscopy. Diameter growth...
protein secretory systems are found in all living organisms including gram negative bacteria, and eukaryotic cell’s organelles that are derived from these bacteria. unlike all other living organisms, gram negative bacteria have different protein secretory systems. at least there are four different independent secretory systems, which are responsible to transport the secreted proteins to the mil...
Bacterial cell-surface display systems coupled with quantitative screening methods offer the potential to expand protein engineering capabilities. To more fully exploit this potential, a unique bacterial surface display scaffold was engineered to display peptides more efficiently from the surface exposed C- and N-termini of a circularly permuted outer membrane protein. Using directed evolution,...
Background: Simultaneous cell surface display and secretion is desirable for protein evolution and selection. Results: Alternative splicing enables simultaneous cell surface display and secretion of the same protein or an alternate form to facilitate screening. Conclusion: Analysis of secreted protein complements cell surface display for evolution of protein function. Significance: This technol...
Ice nucleation protein (INP) is frequently used as a surface anchor for protein display in gram-negative bacteria. Here, MalE and TorA signal peptides, and three charged polypeptides, 6×Lys, 6×Glu and 6×Asp, were anchored to the N-terminus of truncated INP (InaK-N) to improve its surface display efficiency for human Arginase1 (ARG1). Our results indicated that the TorA signal peptide increased ...
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