The temperate bacteriophages lambda and P22 share similarities in their site-specific recombination reactions. Both require phage-encoded integrase (Int) proteins for integrative recombination and excisionase (Xis) proteins for excision. These proteins bind to core-type, arm-type, and Xis binding sites to facilitate the reaction. lambda and P22 Xis proteins are both small proteins (lambda Xis, ...

activity and mRNA expression (NOX1, NOX4, p67, p47, p22). Inhibitors of AP-1 significantly diminished the Ang II or TNF-stimulated p22 promoter activity and protein level. Transient overexpression of c-Jun/c-Fos upregulated p22 promoter activity. Transcription factor pull-down assay and chromatin immunoprecipitation demonstrated the physical interaction of c-Jun protein with predicted AP-1–bind...

The median nerve N20 and P22 SEP components constitute the initial response of the primary somatosensory cortex to somatosensory stimulation of the upper extremity. Knowledge of the underlying generators is important both for basic understanding of the initial sequence of cortical activation and to identify landmarks for eloquent areas to spare in resection planning of cortex in epilepsy surger...

Background—NADH/NADPH oxidase is an important source of superoxide in the vasculature. Recently, we found that polymorphism of the gene p22, a critical component of this oxidase, is associated with a risk of coronary artery disease. The aim of this study was to investigate the localization of p22 in human coronary arteries and to examine its difference in expression between nonatherosclerotic a...

Phage P22 induces a lysozyme in Salmonella typhimurium cells toward the later stage of its multiplication. P22 lysozyme has been purified about lOOO-fold starting from the lysate of C1 (clear plaque-forming mutant of phage P22)infected cells. The enzyme has an optimum pH between 7 and 8 and its activity is dependent on the ionic strength of the assay medium. Salts like MgCl,, NaCI, and KC1 are ...

In a previous study, we found that the p22 subunit of the NADH/NADPH oxidase is overexpressed in vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHRs) with enhanced vascular production of superoxide anion (O2 ). Thus, we have investigated whether changes in the sequence or activity of the promoter region of p22 gene are present in SHRs. To carry out this analysis, fir...

Silica-encapsulated Raman-reporter embedded SERS nanoprobes, named nanoaggregate embedded beads (NAEBs), were conjugated to the Salmonella specific tailspike protein (TSP) isolated from the P22 bacteriophage to enable a highly specific and ultrasensitive optical transduction platform. We demonstrate three successful surface conjugation strategies and highlight the detection of a single bacteriu...