Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by enzymes. RFLP-PCR an easy-to-perform and inexpensive tool for initial analysis SNPs potentially associated with some monogenic diseases, as well in genotyping, genetic mapping, lineage screening, for...

Using locally accessible organic materials as fertilizers in hydroponics can represent a sustainable alternative to the synthetic mineral typically used. This study aimed at developing simple bioponic method using chicken or goat manure on lettuce (Lactuca sativa var. Lucrecia) nutrient film technique, requiring few inputs. The first experiment compared solutions made from droppings and terms o...

Preserved clinical material is a unique source for proteomic investigation of human disorders. Here we describe an optimized protocol allowing large scale quantitative analysis of formalin fixed and paraffin embedded (FFPE) tissue. The procedure comprises four distinct steps. The first one is the preparation of sections from the FFPE material and microdissection of cells of interest. In the sec...

Failure to obtain sufficient material from marrow aspiration (dry tap) posed a diagnostic problem in two patients with pancytopenia. By using collagenase digestion of the trephine biopsy specimen, a precise diagnosis was reached. This technique is very useful because it permits flow cytometric and immunocytochemical analyses of cell suspensions obtained after collagenase digestion of the trephi...

Growing research effort in recent years has focused on the role of 5-methylcytosine (5-MeC) in gene expression. 5-MeC has been implicated in such phenomena as genomic imprinting, maintenance of heterochromatin, and the direct control of gene expression through proteins which bind methylated DNA (1). As interest in 5-MeC has intensified, several new techniques have been developed to find and stu...

Tryptic digestion of proteins is one of the critical steps in any proteomics analysis and may heavily impact the quality of the data generated from the sample. The peptides generated are analyzed by mass spectrometry and used to identify and characterize the proteins in the sampled population. Several factors have been found to influence the results of proteolysis and the subsequent analysis. S...