conclusion: the newly produced recombinant protein bmk revealed to be immunogenic. moreover, anti-bmk antibodies produced in mice were able to recognize both the recombinant bmk neurotoxin and the one in m. eupeus crude venome. taken together, the molecular characterization and recombinant production of the iranian scorpion m. eupeus venom component can serve as a new probe for further studies ...

conclusions these results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal s. aureus in rats. it can be recommended for human clinical trial studies. results recombinant lysostaphin at 100 μg/ml concentration showed a significant decrease of the optical density compared to the control samples. the in vivo study demonstrated that a single dose (200 μg/d...

background: the over-expression of recombinant proteins in large amount is important for production of therapeutic proteins and structural study. there are several systems for expression of recombinant proteins. one of the most relevant expression systems is escherichia coli (e. coli). although this organism has many advantages, most of recombinant proteins expressed in e. coli hosts form inclu...

background: the purity and correct folding of a recombinant protein is critical for any structural, biochemical and vaccine design studies. plasmodium vivax duffy binding protein-ii is a leading vaccine candidate for vivax malaria. in the present study, the purification process of recombinant dbp-ix (a variant form of pvdbp-ii) was optimized to achieve the highest yield and purity. moreover, na...

We evaluated the effect of vaccination with the SAG1 protein of Toxoplasma gondii against congenital toxoplasmosis in mice with different genetic backgrounds. In BALB/c mice (H-2(d)), vaccination reduced the number of infected fetuses by 50% and was associated with a mixed type 1 and type 2 immunity. In CBA/J mice (H-2(k)), vaccination increased the number of infected fetuses by 50% and was ass...

background: the witches’ broom disease of lime caused by candidatus phytoplasma aurantifolia, is the most devastating disease of acidian lime in the southern parts of iran. objectives: at present, no effient method has been developed for controlling the disease, therefore quarantine approaches such as early detection and subsequent eradication of infected trees is very important. toward this ...

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto ...

background: infectious bursal disease virus (ibdv) causes a highly immunosuppressive disease in chickens and is a pathogen of major economic importance to the poultry industry worldwide. the vp2 protein is the major host-protective immunogen of ibdv and has been considered as a potential subunit vaccine against the disease. vp2 coding sequence was cloned in an inducible fungal vector and the pr...

helicobacter pylori infection is among the most prevalent infections in the world involving more than half the adult population. h. pylori infection results in active chronic gastritis, peptic ulcers and enhances the risk of gastric malignancies. it is of utmost importance to prevent h. pylori infection particularly in highly prevalent countries including iran. the urease holoenzyme produced by...