Effective and rapid in vitro plantlet regeneration from protocorms of Cymbidium aloifolium using chitosan, coconut water potato extract were investigated. Two experiments performed twelve week old size 2-3 mm. In the first experiment, propagated on solid New Dogashima (ND) medium supplemented with various concentrations chitosan (0, 0.05, 0.10 1.00 mg/l). For second cultured same 100, 150 200 m...

• Specific orchid–fungal associations are known for nonphotosynthetic orchids but fungal diversity in photosynthetic orchids is thought to be quite broad. Specific fungal associations will figure prominently in conservation efforts, while diverse associations may require less attention. We combined culture techniques with ITS and mtLSU sequences and phylogenetic analysis to determine the geneti...

Abstract One of the obstacles for production Orchidaceae plants is seed propagation. Thus, in vitro cultivation, luminosity and type sealing bottles are factors that influence germination initial development orchids. Therefore, objective was to evaluate light condition, evaluation time Dendrobium nobile Lindl. After sowing, cultures were subjected two irradiance conditions according flasks: 7.0...

The compatibility of mycorrhizal fungi with the early growth stage orchids is essential for their growth. In this study, and promotion effects 14 Tulasnella strains from different hosts were studied by co-culturing them protocorms Dendrobium crepidatum, which has high ornamental economic value in China. ITS–LSU–SSU–TEF combined sequence analysis divided into three clades belonging to calospora ...

Cremastra appendiculata var. variabilis (Blume) I.D. Lund), also known as single-leaf cremastra (SLC), is a rare and threatened species native to Korea, it has the potential be grown beautiful flowering pot or garden plant. There still no reliable strategy multiply SLC. Thus, an effective method for propagating was needed its conservation mass production. In present study, we examined effects o...