Phospholipase C-(gamma) (PLC-(gamma)) is activated in many cell types following growth factor stimulation. Our understanding of the role of PLC-(gamma) in cell growth and differentiation has been severely limited by the dearth of mutations in any organism. In this study, we show that the Drosophila gene small wing (sl), identified by Bridges in 1915, encodes a PLC-(gamma). Mutations of sl resul...

Phosphoinositide-specific phospholipase C (PLC) is dependent on Ca2+ ions for substrate hydrolysis. The role of an EF-hand Ca(2+)-binding motif in Ca(2+)-dependent PLC activity was investigated by site-directed mutagenesis of the Dictyostelium discoideum PLC enzyme. Amino acid residues with oxygen-containing side chains at co-ordinates x, y, z, -x and -z of the putative Ca(2+)-binding-loop sequ...

Phospholipase C-γ (PLC-γ) is activated in many cell types following growth factor stimulation. Our understanding of the role of PLC-γ in cell growth and differentiation has been severely limited by the dearth of mutations in any organism. In this study, we show that the Drosophila gene small wing (sl), identified by Bridges in 1915, encodes a PLC-γ. Mutations of sl result in extra R7 photorecep...

Juvenile hormone (JH) is a key regulator of a wide diversity of developmental and physiological events in insects. Although the intracellular JH receptor methoprene-tolerant protein (MET) functions in the nucleus as a transcriptional activator for specific JH-regulated genes, some JH responses are mediated by signaling pathways that are initiated by proteins associated with plasma membrane. It ...

Panebra, A., S.-X. Ma, L.-W. Zhai, X.-T. Wang, S. G. Rhee, and S. Khurana. Regulation of phospholipase C-g1 by the actin-regulatory protein villin. Am J Physiol Cell Physiol 281: C1046–C1058, 2001.—The actin-regulatory protein villin is tyrosine phosphorylated and associates with phospholipase C-g1 (PLC-g1) in the brush border of intestinal epithelial cells. To study the mechanism of villin-ass...

Eukaryotic phosphoinositide-specific phospholipases C (PI-PLC) specifically hydrolyze phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)], produce the Ca(2+)-mobilizing agent inositol 1,4,5-trisphosphate, and regulate signaling in multicellular organisms. Bacterial PtdIns-specific PLCs, also present in trypanosomes, hydrolyze PtdIns and glycosyl-PtdIns, and they are considered important vir...

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in initiating the surface-mediated signal transduction by generating second messenger molecules, diacylglycerol, and inositol phosphates (see [ 1-41 for reviews). Results accumulated during the last few years clearly indicate that the response of phosphoinositide signalling to a specific stimulus varies depending on tissue and...

Abstract We investigated whether phospholipases play a role in citrus fruit susceptibility to be infected by Penicillium digitatum, and connection exists between hormone abscisic acid (ABA) the fruit-P. digitatum interaction. Changes both activity of enzymes PLD, PLC PLA2 expression set genes encoding them response infection Navelate (Citrus sinensis (L.) Osbeck) orange its ABA-deficient mutant...

BACKGROUND Numerous experimental and epidemiological studies have demonstrated a link between Clonorchis sinensis (C. sinensis) infestation and cholangiocarcinoma (CCA) as well as hepatocellular carcinoma (HCC). The underlying molecular mechanism involved in the malignancy of CCA and HCC has not yet been addressed. Csseverin, a component of the excretory/secretory products of C. sinensis (CsESP...

Volume overload due to arteriovenous (AV) shunt results in cardiac hypertrophy followed by the progression to heart failure. The phosphoinositide phospholipase C (PLC) converts phosphatidylinositol 4,5-bisphosphate (PIP(2)) to 1,2-diacylglycerol (DAG) and inositol (1,4,5)-trisphosphate (IP(3)), which are known to influence cardiac function. Therefore, we examined the time course of changes in D...