Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Transferase (GST)) were engineered on the N-terminus of mature lysyl oxidase. Total enzyme yields were determined to be 1.5 mg for the Nus-A tagged e...

Pix is a plant growth regulators thatinterfereswithotherhormones and affectthegerminationandgrowthof cotton.In thisstudythe effect ofdifferentconcentrationsof pix, namely, 0 (control),10, 20, 30and40 ppm on germination,radicledry weight, radiclelength,insolubleandsoluble sugarsandantioxidantenzymes activity (catalase, peroxidase and poly phenol oxidase) incottonseedling (Gossypium hirsutum L.cv...

Previous studies from this laboratory (1) have demonstrated the existence of several different types of “xanthine oxidase” in nature. The enzymes in milk and mammalian tissues appear to be quite similar, but can be differentiated by the inhibitor Antabuse, which affects only the tissue enzyme (2). Antabuse can also be used to separate the dehydrogenase and oxidase activities of tissue xanthine ...

Xanthine oxidase is molybdenum and iron-containing flavoprotein, catalyzing the final oxidation stage of purines oxidative transformation pterins some aliphatic aromatic aldehydes. Despite importance this enzyme, distribution xanthine in traditional household animal’s milk tissues unknown. Formerly, we have found most molecules animal are inactive because a lack molybdenum. Ovine was processed ...

Vanillyl-alcohol oxidase (EC 1.1.3.7) from Penicillium simplicissimum was modified with p-mercuribenzoate. One cysteine residue reacts rapidly without loss of enzyme activity. Three sulfhydryl groups then react in an 'all or none process' involving enzyme inactivation and dissociation of the octamer into dimers. The inactivation reaction is slowed down in the presence of the competitive inhibit...

Previous reports on rabbit liver aldehyde oxidase described the purification and some properties of the enzyme (1) and the differential inhibition of electron transfer to various acceptors (2). The latter study furnished evidence for the participation of several electron carriers in the internal electron transport sequence of the enzyme. The present communication summarizes the results of studi...

The composition of milk xanthine oxidase has been reinvestigated. When the enzyme is prepared by methods that include a selective denaturation step in the presence of sodium salicylate the product is obtained very conveniently and in high yield, and is homogeneous in the ultracentrifuge and in recycling gel filtration. It has specific activity higher than previously reported preparations of the...

During senescence of broccoli (Brassica oleracea L. var. italica) florets (flower buds), the rate of ethylene production increased, almost paralleling the rapid increase in 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity. The increase in ACC oxidase activity was strongly suppressed by the administration of cycloheximide, suggesting that it may have resulted from the de novo synthesis o...

NADH oxidase (EC 1.6.99.3) was purified from cell lysates of Serpulina (Treponema) hyodysenteriae B204 by differential ultracentrifugation, ammonium sulfate precipitation, and chromatography on anion-exchange, dye-ligand-affinity, and size-exclusion columns. Purified NADH oxidase had a specific activity 119-fold higher than that of cell lysates and migrated as a single band during denaturing ge...

Diamine oxidase was previously measured in human pregnancy serum with putrescine or histamine as substrate. We have now documented the presence of spermidine oxidase activity in pregnancy serum by means of a specific radioactive assay with l '4Clspermidine as substrate and Dowex 50 cation-exchange chromatography to separate products from substrate. The apparent Km of a partially purified prepar...