In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30 microg-cefoxitin and 1 microg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. ...

Accurate detection of methicillin resistance (MR) in coagulase negative staphylococci (CoNS) is of utmost importance as infections due to CoNS are on the rise. Various phenotypic methods have been described for detecting mecA mediated MR with varying performance characteristics. The present study was designed to evaluate the performance of oxacillin and cefoxitin disc diffusion / MIC test and o...

The performance characteristics of the E-test (AB Biodisk, Solna, Sweden), the ATB Staph, the Rapid ATB Staph, and the Vitek GPS-503 card (bioMérieux, La Balme Les Grottes, France) methods for the detection of oxacillin resistance in a collection of staphylococci with a high proportion of troublesome strains were evaluated. Sixty-four Staphylococcus aureus strains and 76 coagulase-negative stap...

The Mastalex MRSA latex agglutination method was evaluated with 52 methicillin-resistant and 27 methicillin-susceptible strains of Staphylococcus aureus grown on various media. All tests were correct with colonies grown on blood agar with or without oxacillin 2 mg/L. Tests on colonies grown on mannitol salt agar were less reliable, although addition of oxacillin 2 mg/L improved performance. One...

OBJECTIVE To determine, by phenotypic and genotypic methods, oxacillin susceptibility in Staphylococcus aureus strains isolated from pediatric and neonatal intensive care unit patients seen at the University Hospital of the Botucatu School of Medicine. METHODS A total of 100 S. aureus strains isolated from the following materials were studied: 25 blood cultures, 21 secretions, 12 catheters, 3...

Non typhoidal salmonellosis (NTS) is on the rise in many countries. Most common causes of NTS are Salmonella enterica serovars Typhimurium, Enterica, Newport and Heidelberg and typically present as self limiting gastroenteritis. However, in immunocompromised individuals and children, complications like bacteremia and focal metastatic infections can ensue. Antibiotic resistance is also of concer...

BACKGROUND Apart from being a major cause of mortality, nosocomial infections due to Staphylococcus aureus have been imposing a burden on patients, hospitals and health care systems. The present study was designed to determine the prevalence of methicillin resistant S. aureus (MRSA) among nosocomial isolates along with their phenotypic characterization. METHODOLOGY MRSA and methicillin sensit...

discussion our results indicated that agar screening was superior to oxacillin disk agar diffusion. a comparison between the antibiotic sensitivity patterns of the mrcns and the methicillin-susceptible coagulase-negative staphylococci (mscns) showed that the mrcns were predominantly multiple-drug resistant isolates as the simultaneous resistance rate to 4 or more antibiotics in the mrcns and ms...

PURPOSE To evaluate different methods of oxacillin susceptibility testing of ocular isolates, considering polymerase chain reaction (PCR) as the 'gold standard', and to compare the in vitro susceptibility to oxacillin with that of other antimicrobials used in ophthalmologic practice. METHODS The Vitek gram-positive identification card was used to identify ocular coagulase negative Staphylococ...