Since ovarian cancer cells express CD44, which causes very strong cell adhesion to peritoneal mesothelium and an unfavourable prognosis, we designed small interfering RNA (siRNA) targeting the CD44 gene to analyse the functional consequences of this inhibition in human ovarian cancer. We transfected ovarian cancer cell line SKOV-3 with well-designed CD44 siRNA or control siRNA. Western blot ana...

AIM Evaluation of the laparoscopic assisted revision of the ventriculoperitoneal shunt as an alternative technique to the traditional laparotomy. MATERIAL AND METHODS Out of 1181 elective neurosurgical procedures have been done from January 2009 to December 2010. There are 98 (8.2%) ventriculoperitoneal shunt procedures. New shunt insertion was performed 41 times (41.9%) a...

Results Overall, 1790 patients showed no post-operative complications and were assigned to the non-complication group, whereas 27 patients suffered from IAA postoperatively and were assigned to the IAA group. The incidence of IAA after LA was 1.4% and the only identified risk factor for IAA was dirty fluid collection in the peritoneal cavity observed during the operation. (P < 0.001) On logisti...

In spite of the approaches that have been proposed to reduce postoperative peritoneal adhesions, they remain a major clinical problem because of the associated intestinal obstruction, chronic pelvic pain, female infertility and difficulties at the time of reoperation. The pathogenesis of the process have been focused almost exclusively on the local events induced by the surgical trauma, and the...

Whether induced by infection, inflammation, ischemia, and/or surgical injury, peritoneal adhesions are the leading cause of pelvic pain, bowel obstruction and infertility. It is clear that while postsurgical peritoneal wounds heal without adhesions in some patients, others develop severe scarring from seemingly equal procedures; in addition, in the same patient, adhesions can develop at one sur...

Extravascular coagulation leading to fibrin deposition accompanies many immune and inflammatory responses. Although recognized by pathologists for decades, and probably pathologic under certain conditions, the physiologic functions of extravascular coagulation remain to be fully defined. This study demonstrates that thrombin can activate macrophage adhesion and prompt interleukin-6 (IL-6) and m...

background :although  laparoscopic   cholecystectomy  is   associated  with  less   post-operative  pain compared  to   open  cholecystectomy,  diffuse  shoulder  and  abdominal  pain  is   one    of   the    main postoperative complications in  this technique. the  aim  of this study was  to  compare the  abdominal and shoulder pain after laparoscopic cholecystectomy by  applying intra-abdomin...

OBJECTIVE Peritoneal adhesions are almost ubiquitous following surgery. Peritoneal adhesions can lead to bowel obstruction, digestive problems, infertility, and pain, resulting in many hospital readmissions. Many approaches have been used to prevent or treat adhesions, but none offer reliable results. A method that consistently prevented or treated adhesions would benefit many patients. We hypo...

BACKGROUND AND STUDY AIMS There have been concerns about peritoneal seeding after endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) of mucinous pancreatic cystic lesions. The aims of this study were to determine the frequency of postoperative peritoneal seeding in patients with intraductal papillary mucinous neoplasm (IPMN) who had undergone pre-operative EUS-FNA and to compare it w...

in our experimental study, 48 rats were divided into six groups in a randomized manner: a (1-3) and b (1-3) groups. a1 and b1 were negative controls. laparotomy was performed and peritoneal inflammation on exposed bowel and peritoneum in a (1-3) and experimental infection induced in peritoneal cavity of b (1-3). each rat of a2 and b2 received 100 iu intraperitoneal standard heparin and each rat...