This study presents the effects of prenatal ethanol exposure on the morphology of the seminiferous tubules of the testes in the adult male rat. Timed-pregnant adult female Wistar rats (average weight 200 g) were given daily intragastric intubation of 5.8 g/kg ethanol between gestation days 9 and 12. Pair-fed and ad lib-fed animals served as controls. The pups were weighed at birth and weaned at...

Lewisite, a potent toxic vesicant and chemical warfare agent, is used in a number of research laboratories, is stored in large quantities at depot sites throughout the USA and is occasionally transported to distant sites. Thus, the potential for environmental or occupational exposure exists where lewisite is present. A 42-week two-generation study was conducted to determine the reproductive con...

Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient mode...

Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. To recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were e...

Prenatal caffeine exposure adversely affects the development of the reproductive organs of male rat offspring. Thus, it is conceivable that peripubertal caffeine exposure would also influence physiologic gonadal changes and function during this critical period for sexual maturation. This study investigated the impact of high doses of caffeine on the testes of prepubertal male rats. A total of 4...

The use of non-ejaculated sperm coupled with intracytoplasmic sperm injection has become a globally established procedure for couples with azoospermic male partners who wish to have biological offspring. Surgical methods have been developed to retrieve spermatozoa from the epididymides and the testes of such patients. This article reviews the methods currently available for sperm acquisition in...

Ambient black carbon (BC), a main constituent of atmospheric particulate matter (PM), is primary particle that mainly generated by the incomplete combustion fossil fuel and biomass burning. BC has been identified as potential health risk via exposure. However, adverse effects exposure to on male reproductive system remain unclear. In present study, we explored maternal oxidized (OBC) during pre...

Dioxins are persistent organic pollutants interfering with endocrine systems and causing reproductive and developmental disorders. The objective of our project was to determine the impact of an in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on reproductive function of male and female offspring in the rat with a special emphasis on the immature period. We used a low dose of TCDD...

Dietary bisphenol A (BPA) was evaluated in a mouse two-generation study at 0, 0.018, 0.18, 1.8, 30, 300, or 3500 ppm (0, 0.003, 0.03, 0.3, 5, 50, or 600 mg BPA/kg/day, 28 per sex per group). A concurrent positive control group of dietary 17beta-estradiol (0.5 ppm; 28 per sex) confirmed the sensitivity of CD-1 mice to an endogenous estrogen. There were no BPA-related effects on adult mating, fer...