The nicotinamide adenine dmucleotide-specific isocitrate dehydrogenase of yeast was reported by Kornberg and Pricer (1) to have an absolute requirement for adenylic acid and not to catalyze the reverse reaction under conditions suitable for such catalysis by the nicotinamide adenine dinucleotide phosphatespecific enzyme. Similar results have been obtained for the nicotinamide adenine dinucleoti...

As part of an investigation of the growth of tomato plants supplied with either nitrate or ammonium nitrogen (6), leaves were assayed for NAD4 and NADP. Anderson and Vennesland (1) assayed NAD and NADP in various green tissues and reported recoveries between 50 % and 85 % of NAD and NADP added to spinach leaf extracts. Very low levels of NAD and NADP were recorded for tomato leaf extracts. Thes...

glucose 6-phosphate dehydrogenase (g6pd) from streptomyces aureofaciens was purified and denatured in 6 m urea. denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...

Introduction Oxidative stress seems to be a role in the atherosclerosis process, but research in human beings is scarce. Objective To evaluate the role of oxidative stress on human aortas of patients submitted to surgical treatment for advanced aortoiliac occlusive disease. Methods Twenty-six patients were divided into three groups: control group (n=10) formed by cadaveric organ donors; sev...

Nicotinamide adenine dinucleotide phosphate oxidase, a key superoxide-producing enzyme, plays a critical role in microglia-mediated chronic neuroinflammation and subsequent progressive dopaminergic neurodegeneration in Parkinson's disease. Although nicotinamide adenine dinucleotide phosphate oxidase-targeting anti-inflammatory therapy for Parkinson's disease has been proposed, its application i...

Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...

Chain breakage in DMA induced by bleomycin A (BLM) was enhanced more than 150-fold by the reduced nicotinamide adenine dinucleotide phosphate-dependent elec tron transport system of rat liver microsomes. However, the enhancement effect on DMA was partially reduced by the preincubation of BLM with the microsomal systems. BLM-Cu2' was found to stimulate considerably micro somal reduced nicotinami...

Many biological assays have as their basis a link to the oxidative status of nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP). Many dehydrogenase enzymes use these coenzymes to transfer hydrogen groups between molecules. Because the reduced forms of these molecules differ from the oxidized forms in their ability to absorb light, it is possible to qua...

Many biological assays have as their basis a link to the oxidative status of nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP). Many dehydrogenase enzymes use these coenzymes to transfer hydrogen groups between molecules. Because the reduced forms of these molecules differ from the oxidized forms in their ability to absorb light, it is possible to qua...

Exogenous nicotinamide adenine dinucleotide is not utilized per se by Escherichia coli, but is converted to nicotinamide and thence to nicotinamide adenine dinucleotide via nicotinate.