Chinda pepper (Capsicum annuum L.) is classified as a large chili that an important economic crop of Thailand. The substance in the responsible for its spicy taste capsaicin. Presently, capsaicin peppers has been extracted use food and medical products. This research compared content from dried four different solvents: H2O, acetone, ethanol (95% v/v), binary solvent acetone water at ratio 1:1 v...

Accurate DNA quantification is a highly important method within molecular biology. Methods widely used to quantify are UV spectrometry and fluorometry. In this research, seven different samples one blank (MilliQ ultrapure water) were quantified by three analysts using spectrophotometric (i.e., NanoDrop instrument) fluorometric the AccuGreen High Sensitivity kit, AccuClear Ultra Qubit dsDNA HS A...

The breakup pathway of Rayleigh fission a charged drop is unequivocally demonstrated by first its kind, continuous, high-speed imaging levitated in an AC quadrupole trap. experimental observations consistently exhibited asymmetric, sub-critical with upward (i.e. opposite to the direction gravity) ejection jet from drop. These experiments supported numerical calculations show that gravity induce...

Optimized and reliable DNA/RNA extraction protocols are a vital tool in clinical practice the context of molecular testing. Here, we present our successful attempt to enhance quantity RNA isolated from specimens, which originally found challenging (breast testis). We compared several purification methods with special focus on two AllPrep system-based (QIAGEN). Our data suggest that addition pro...

Extraction of DNA is very important nowadays in bio-molecular researches. Extracted DNA should be purified and the quality of DNA should also be very high. The objective of the study was to develop a simple efficient method to isolate DNA from the rice varieties in an open laboratory environment, and to eliminate the usage of expensive chemicals and tools. The DNA extraction methods developed b...

Direct isolation of soil DNA comes as an emerging technology to understand the microbial diversity of a particular environment circumventing the dependency on culturable methods. Soil DNA isolation is tough due to the presence of various organic components present in soil which interfere in extraction procedure. Here, we report a novel direct soil DNA extraction protocol utilizing bare superpar...

This work pertains to the method for modification of silicon (Si) wafer morphology by metal-assisted chemical etching (MacEtch) technique suitable for fabrication of antireflective Si surfaces. For this purpose, we made different Au catalyst patterns on the surface of Si substrate. This modification allowed to obtain the close-packed Au nanodrop (ND) pattern that generates the nanowires (NWs) a...

Objective: To determine the molecular diversification and frequency of mecA Gene in MRSA infections hospitalised patients at Islamabad, Pakistan.
 Study design: Cross-sectional study.
 Place Duration: Pakistan Institute Medical Science, Pakistan, from Sep 2017 to 2018.
 Methodology: explore occurrence pathogenic S. aureus 500 samples were collected. All isolates further character...