For the first few days of cultivation of connective tissue in hypertonic, hypotonic, and diluted medium, cell proliferation was stimulated. The first outgrowths of new cells in the modified media did not occur sooner than in normal medium. In hypertonic medium the density of the area of cell proliferation appeared to be greater than the control, but in hypotonic or diluted medium there seemed t...

Development of MMRS (modified MRS Agar) culture medium; the absolute vegetarian culture medium containing plant seed powder in place of beef extract for the growth of probiotics. Vegetarian probiotic foods by definition must be free from all animal-derived ingredients. This research provides an alternative efficacious, cost effective vegetal culture medium. The culture medium developed in prese...

The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expresse...

BACKGROUND & OBJECTIVES In human immunodeficiency virus (HIV) infected persons with pulmonary tuberculosis (TB), sputum may not always show acid fast bacilli (AFB). Moreover, in most cases of suspected extrapulmonary TB (irrespective of HIV status) mycobacteria-containing material is not readily available for investigation. This study evaluated whether blood culture for Mycobacterium tuberculos...

The selectivity of a range of culture media for the detection of Salmonella was assessed using 435 strains of gram-negative bacteria. These media showed limited ability to inhibit non-Salmonella strains found in stool samples. We report the evaluation of alafosfalin as a selective agent for isolation of Salmonella from stool samples. Susceptibility studies with this agent showed that non-typhi ...

The objective of this study is to investigate the efficiency of calcium carbonate bioprecipitation by Lysinibacillus sphaericus, Bacillus subtilis and Pseudomonas putida, obtained from the Coleção de Culturas do Instituto Nacional de Controle de Qualidade em Saúde (INCQS), as a first step in determining their potential to protect building materials against water uptake. Two culture media were s...

among pome fruit trees, quince is the most susceptible tree to fire blight. following selection of several promising quince cultivars, this research aimed at optimization of in vitro propagation protocol of the selected materials. therefore, several experiments were carried out for determination of the best culture medium and plant growth regulators for establishment, proliferation and root ind...

Cotyledonary node explants excised from 21 day old seedlings of T. arjuna produced multiple shoots when cultured on full strength MS or modified MS (1/2 strength major salts and Fe-EDTA) medium supplemented with different concentrations (0.1-1.0 mg/l) of BAP. Maximum 8.9 shoots/explant could be recorded after 30 days of inoculation on modified MS medium supplemented with BAP (0.5 mg/l). A proli...

Commercial culture media present interlot variations in biological activity. We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medium. Furthermore, the medium is stable for several months when stored frozen or refrigerated. The...