Pseudomonas stutzeri strain YC-YH1, isolated from pesticide-polluted soil, efficiently degrades organophosphorus pesticides (OPPs) such as chlorpyrifos, parathion-methyl, triazophos, and parathion. Here, we report the genome sequence (4.83 Mb) of P. stutzeri YC-YH1 to facilitate further investigation of the OPP-degrading mechanism.

Eleven large-plot trials were located across nine counties in eastern Arkansas to evaluate the efficacy of select insecticides on the rice stink bug. Lambda-cyhalothrin, zeta-cypermethrin, methyl parathion, and carbaryl achieved initial control. However, only lambda-cyhalothrin and zeta-cypermethrin maintained significantly lower counts of the rice stink bug at 14 days after treatment (DAT). La...

The effect of sub-lethal doses of Malathion and Methyl-Parathion was studied on the rat liver enzymes. Intravenous administration of both insecticides at weekly interval for four weeks resulted in increase in weight of various tissues, i.e., heart and spleen. Short term (24 hr) and long term (4 weeks) treatment with insecticides resulted in increased specific activities of liver enzymes, Acid p...

A degradative bacterium, M6, was isolated and presumptively identified as Plesiomonas sp. strain M6 was able to hydrolyze methyl parathion to p-nitrophenol. A novel organophosphate hydrolase gene designated mpd was selected from its genomic library prepared by shotgun cloning. The nucleotide sequence of the mpd gene was determined. The gene could be effectively expressed in Escherichia coli.

A simple and rapid method was developed for determination of benomyl, diphenyl (DP), o-phenylphenol (OPP), thiabendazole (TBZ), chlorpyrifos, methidathion, and methyl parathion in whole oranges. These compounds were extracted from a mixture of samples and anhydrous sodium acetate with ethyl acetate. The ethyl acetate extract was concentrated and cleaned up by passing through tandem solid-phase ...

Concentrations of organophosphate pesticides, including chlorpyrifos and methyl parathion, were measured in ambient air breathed by farmers in Tambon Bang Rieng, Thailand. An exposure assessment and comparison was made between the levels of pesticide exposure of traditional and integrated pest management (IPM) farmers. Thirty-three air samples were collected during pesticide spraying. Tradition...

Unravelling the role of neuromessenger processes in learning and memory has long interested researchers. We investigated the effects of an acetylcholinesterase blocker, Methyl Parathion (MeP), on honeybee learning. We used visual and olfactory tasks to test whether MeP had a detrimental effect on the acquisition of new knowledge when this new knowledge contradicts previously acquired one. Our r...

Pichia pastoris is commonly used to express and secrete target proteins, although not all recombinant proteins can be successfully produced. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as a model to study the importance of the N-terminus of the protein for its secretion. While MPH can be efficiently expressed intracellularly in P. pastoris, it is not secre...