MetHb is often defined as oxidised Hb; that is the haem iron is oxidised from the ferrous (Fe) state to the ferric (Fe) state. In addition, in metHb the sixth coordination site of the haem iron is liganded to a water molecule; this shifts the absorbance maxima from that of Hb causing metHb to be chocolate brown in colour (acid form). At pH ~8 the water molecule is replaced by a bound hydroxyl g...

Fluorescence than Ferrous Hb In the absence of haemoglobin (Hb) products, no cisparinaric Acid (PnA) fluorescence is lost. However, upon addition of ferrous Hb, there is an increasing amount of fluorescence lost. Substantial fluorescence quenching is seen on the addition of extracellular mHb, with significantly less using an intracellular product. This suggests that the cellular membrane is an ...

in the form of methaemoglobin, but was apparently accounted for as carboxyhaemoglobin after the addition of carbon monoxide and dithionite. 4. The green pigment is distinct from choleglobin, and its formation is not prevented by carbon monoxide, showing that oxyhaemoglobin is not an intermediate in the reaction. We wish to thank Mr B. A. Collett for technical assistance during a part of the work.

The objective of the present study was to determine whether β1-4 galacto-oligosaccharides (GOS) and Candida kefyr combined with nitrate as manipulators could suppress rumen methanogenesis without nitrate poisoning in sheep. Four rumen fistulated wethers were allocated to a 4×4 Latin square design. Nitrate (1.3 g NaNO3 kg body weight) with and without GOS and Candida kefyr were administered into...

BACKGROUND & OBJECTIVE Liver cirrhosis is associated with gastrointestinal haemorrhage and oesophageal variceal bleeding. Altered platelet functions has been reported to be a cause of bleeding complication. We carried out this study to find out the level of oxidative stress in the red blood cells of patients with liver cirrhosis. METHODS Fifty patients admitted with the complication of liver ...

Soot is a mass of impure particles carbon obtained from incomplete hydrocarbon combustion. an ultrafine air-borne pollutant and enters into the body through ingestion, skin contact inhalation causing devastating effects on blood cells. The aim study was to determine effect soot methaemoglobin oxyhaemoglobin levels individuals resident in both Iwofe, Port Harcourt (exposed subjects) Ihiala, Anam...

The time course of haemoglobin autoxidation was studied under various conditions at 37 degrees C, and the changes in oxyhaemoglobin, intermediate haemoglobins and methaemoglobin during the reaction were analysed by isoelectric focusing on Ampholine/polyacrylamide-gel plates. Under various conditions (10 mM-phosphate buffer, 10 mM-phosphate buffer with 0.1 M-phosphate buffer, 10 mM-phosphate buf...