Bacillus sp. MG-33 was isolated from the desert of Rajasthan (India). The organism produced 500 and 200 Ug of thermostable mannanase (after 96h) in solid state fermentation (SSF) of wheat bran and wheat straw rich-soda pulp at the moisture ratio of 1:1.5 and 1:3 at 30oC, respectively. Two-step partially purified mannanase was optimally active at 65oC and was 100% thermostable at 55 to 60oC for ...

in order to study the effect of different levels of β-mannanase enzyme on the performance, blood parameters and carcass characteristics of broiler chicks (arbor acres), an experiment was conducted with 240 checks (44.6 grams average weight) under a completely randomized design with 4 treatments and 5 replications and 12 checks per each replicate. β-mannanase enzyme with the levels of zero (t1 c...

A DNA genomic library constructed from Bacillus stearothermophilus, a gram-positive, facultative thermophilic aerobe that secretes a thermostable b-mannanase, was screened for mannan hydrolytic activity. Recombinant b-mannanase activity was detected on the basis of the clearing of halos around Escherichia coli colonies grown on a dye-labelled substrate, Remazol brilliant blue-locust bean gum. T...

β-Mannanases hydrolyze mannan-based hemicelluloses and liberate short β-1,4 manno oligomers, which can be further hydrolysed to mannose by β-mannosidases. Such enzymes are not only of academic interest but also they have potential biotechnological applications in a wide range of industrial enzyme market, including food and feed technology, coffee extraction, bioethanol production, slime control...

A DNA genomic library constructed from Bacillus stearothermophilus, a gram-positive, facultative thermophilic aerobe that secretes a thermostable beta-mannanase, was screened for mannan hydrolytic activity. Recombinant beta-mannanase activity was detected on the basis of the clearing of halos around Escherichia coli colonies grown on a dye-labelled substrate, Remazol brilliant blue-locust bean ...

BACKGROUND Lactobacillus plantarum is considered as a potential cell factory because of its GRAS (generally recognized as safe) status and long history of use in food applications. Its possible applications include in situ delivery of proteins to a host, based on its ability to persist at mucosal surfaces of the human intestine, and the production of food-related enzymes. By displaying differen...

The present study was aimed to investigate the effects of supplemental β-mannanase on in vitro ileal disappearance (IVID) and total tract (IVTTD) dry matter (DM) barley, canola meal, copra corn, corn distillers dried grains with solubles, cottonseed palm kernel soybean wheat for pigs. Feed ingredient samples were finely ground divided into control group treatment group. prepared contain 990 g/k...

An a-galactosidase and a p-mannanase produced by the hyperthermophilic bacterium, Thermotoga neapolitana 5068 (TN50681, separately and together, were evaluated fortheir ability to hydrolyze guar gum in relation toviscosity reduction of guar-based hydraulic fracturing fluids used in oil and gas well stimulation. In such applications, premature guar gum hydrolysis at lower temperatures before the...

Commercially available enzymes do not contain all the necessary softwood-specific accessory enzymes to obtain high saccharification efficiency. In this work, six saprophytic fungi obtained from Pinus radiata plantation site were screened for the putative softwood-specific accessory enzyme, β-mannanase. A Penicillium sp. was found to produce β-mannanase in both solid (31.6 units/g of dry biomass...

BACKGROUND The methylotrophic yeast, Pichia pastoris, offers the possibility to generate a high amount of recombinant proteins in a fast and easy way to use expression system. Being a single-celled microorganism, P. pastoris is easy to manipulate and grows rapidly on inexpensive media at high cell densities. A simple and direct method for the selection of high-producing clones can dramatically ...