Enzymatic transesterification of soybean oil with methanol and ethanol was studied. Of the nine lipases that were tested in the initial screening, lipase PS from Pseudomonas cepacia resulted in the highest yield of alkyl esters. Lipase from Pseudomonas cepacia was further investigated in immobilized form within a chemically inert, hydrophobic sol-gel support. The gel-entrapped lipase was prepar...

The development of lipoprotein lipase has been examined during adipose conversion of preadipocyte Ob17 cells. These cells have been previously shown to differentiate in clusters of fat cells. The lipoprotein lipase activity, which increases 20-50-fold during adipose conversion, is fully inhibited by anti-lipoprotein lipase y-globulins (directed against the rat heart enzyme) and is heparin-relea...

The combination of the ionic liquid co-lyophilized lipase and microwave irradiation was used to improve enzyme performance in enantioselective esterification of α-lipoic acid. Effects of various reaction conditions on enzyme activity and enantioselectivity were investigated. Under optimal condition, the highest enantioselectivity (E = 41.2) was observed with a high enzyme activity (178.1 μmol/h...

Microbial enzymes are important tools that frequently used in the field of biotechnology. These microbial enzymes, which play a role intracellular biological reactions, many different industries. Lipase, proteases and amylases members hydrolytic enzymes. Lipase enzyme, has most common usage area among is an enzyme catalyzes hydrolysis ester bonds between lipid molecules. The activity lipase com...

Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30°C for 96h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipase was purified 2.56-fold to electrophoretic homo...

Equilibrium-binding data of highly purified 125I-labeled avian lipoprotein lipase to cultured avian adipocytes demonstrate the presence of a class of high affinity binding sites. Analysis of the binding function yielded an association constant of 0.62 x 10(8)M-1 and a maximum binding capacity of 2.1 micrograms/60-mm dish. From a time course of dissociation of 125I-lipoprotein lipase from adipoc...

This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilizati...

Biodegradation of polyesters driven by enzymes is considered as one the most effective way degradation these materials, a to control plastics accumulation in environment. In this study, we present two different strategies tune enzymatic PBS films triggered lipase from Pseudomonas cepacia. Firstly, kinetics was regulated applying multilayer coats polysaccharide alginate and chitosan (Alg/Chi) fi...

BACKGROUND AND OBJECTIVES Lipases are particularly important due to the fact that they specifically hydrolyze acyl glycerol, oils and greases, which is of great interest for different industrial applications. MATERIALS AND METHODS In this study, several lipase-producing bacteria were isolated from wastewater of an oil processing plant. The strain possessing the highest lipase activity was ide...

Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl)adenine in dimethylformamide with different vinyl esters (acetate, butyrate, decanoate, laurate, palmitate). The transesterification activities of these immo...