Activity of L-threonine aldolase in rat liver cytosolic extract was not affected by the omission of alcohol dehydrogenase in a previously established NADPH-linked alcohol dehydrogenase-coupled assay. The liver extract was able to catalyse the dehydrogenation of NADPH with either acetaldehyde (a product of L-threonine aldolase action) or 2-oxobutyrate (a product of L-threonine dehydratase action...

None of the remaining 67 isolates utilized aminoacetone when washed suspensions of the bacteria grown on L-threonine were incubated under standard conditions (Higgins et al., 1968). It was concluded that none of the 83 isolates metabolized L-threonine via aminoacetone. Additional work (S. C. Bell & J. M . Turner, unpublished work) suggests that all the selected isolates possessing high L-threon...

The biosynthesis of l-isoleucine proceeds via a highly regulated reaction sequence connected with l-lysine and l-threonine synthesis. Using defined genetic Corynebacterium glutamicum strains characterized by different fluxes through the homoserine dehydrogenase reaction, we analyzed the influence of four different ilvA alleles (encoding threonine dehydratase) in vectors with two different copy ...

We compared the transcriptome, proteome, and nucleotide sequences between the parent strain Escherichia coli W3110 and the L-threonine-overproducing mutant E. coli TF5015. DNA macroarrays were used to measure mRNA levels for all of the genes of E. coli, and two-dimensional gel electrophoresis was used to compare protein levels. It was observed that only 54 of 4,290 genes (1.3%) exhibited differ...

Bio-cyanidation, as a sustainable and effective method to extract gold from primary secondary resources, has attracted attention because of its environmental friendliness economic benefits. The effect amino acids on bio-cyanide production using Bacillus megaterium (B. megaterium) is less explored area in this field the main interest study. Here, glycine, threonine, glutamine over concentration ...

There is an overlap of carrier-mediated L-amino acid transport and apparent simple diffusion when measured in intestinal brush border membrane vesicles. Using L-threonine and L-glutamine as representative amino acids, this study was undertaken to estimate apparent simple diffusion of L-amino acids and to establish the effective dosage of HgCl2 for completely blocking carrier-mediated L-amino ac...