نتایج جستجو برای: issr markers
تعداد نتایج: 223341 فیلتر نتایج به سال:
Necrophagous fly is the most common insect evidence collected during a death investigation. We analyzed the DNA polymorphism among the five forensically important fly species, namely, Lucilia sericata, Aldrichina grahami, Chrysomya megacephala, Parasarcophaga crassipalpis and Musca domestica using inter simple sequence repeat (ISSR) method. Nine ISSR primers selected from 18 primers could ampli...
Estimation of genetic diversity and evaluation of plant germplasm is the most important step in collection and management of plant genetic resources. Also, comparison of different DNA-based genetic markers in diversity evaluation and then advising the most efficient markers is very important. In order to investigate genetic variation among Persian oak (Quercus brantii Lindi.) populations of Lor...
Collection and conservation of germplasm, particularly for landraces of important agronomically species and organizing their information, cause to continuity of their usefulness and reduce the risk of their genetic erosion. In this experiment, 10 Iranian wheat landraces along with the Chinese Spring cultivar, as control, were used to assess inter and intra populations variation by using ISSR mo...
Intersimple sequence repeat (ISSR) markers were evaluated in order to identify informative markers associated with drought tolerance indices in bread wheat (Triticum aestivum L.) genotypes. Eighteen ISSR primers amplified 92 loci among 20 bread wheat genotypes. Polymorphic information content (PIC) ranged from 0.46 (UBC-857, UBC-864, UBC-867, is9) to 0.21 (is7), with an average of 2.05. Stepwis...
Problem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, using RAPD and ISSR...
Morpho-agronomic and molecular (RAPD and ISSR markers) data were used to evaluate genetic distances between papaya backcross progenies in order to help identify agronomically superior genotypes. Thirty-two papaya progenies were evaluated based on 15 morpho-agronomic characteristics, 20 ISSR and 19 RAPD primers. Manhattan, Jaccard and Gower distances were used to estimate differences based ...
Twenty-seven accessions of Lippia alba Mill. collected in Rio Grande do Sul state, Brazil, were analysed by ISSR and RAPD markers to evaluate their genetic variability and relationships. Six ISSR primers and four RAPD primers generated 120 amplified fragments, most of which were polymorphics. The overall genetic variability among accessions was very high when compared with other plant species. ...
The recently developed Inter-Simple Sequence Repeat PCR (ISSR-PCR) or microsatellite primed PCR or Simple Sequence Repeat (SSR)-Anchored PCR technique detects polymorphic markers in a wide variety of genomes. Usually the ISSR primers are either 5' end-labeled with gamma[32P]ATP or one of the alpha[32P] labeled dNTPs is added to the PCR reaction and the PCR products are resolved on PAGE and auto...
The present study aims at detecting molecular markers, based on RAPD, ISSR and AFLP, for the salttolerant wild barley Hordeum spontaneum as a little is known about its genetic structure and function. Barley is one of the most important cereal crops all over the world. Therefore, the identification of molecular markers for the salttolerant wild species is crucial for the future development of to...
Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید