in this study the effect of salt stress on banding patterns of superoxide dismutase (sod), catalase (cat) and peroxidase (pox) in 18 genotypes of white common bean (phaseolus vulgaris l.) were examined under two levels of nacl )0 and 400 mm) stress. for each isozymic band the “density × area” scores on gels were evaluated by mcid software and considered as enzymatic activity. three isozymes wer...

The nucleotide sequence of a clone containing mouse carbonic anhydrase (CA) eDNA in pBR322 has been determined. The cloned eDNA contains all of the coding region except for nueleotides specifying the first eight amino acids, and all of the 3' noncoding region, which consists of 700 nucleotides. A eDNA clone was identified which contains an additional 54 bp at the 5' end, so that the complete am...

Plastid and cytosolic isozymes of ATP:fructose 6-phosphate 1-phosphotransferase (PFK(p) and PFK(c), respectively) have been isolated from leaves and developing endosperm tissues of the castor oil plant (Ricinus communis L). Endosperm PFK(p) has been purified to apparent homogeneity. Polyclonal antibodies raised against one of the four polypeptides associated with potato tuber PFK (molecular mas...

A histochemical method for distinguishing isozymes of myosin in rat ventricles has been developed. The procedure involves preincubation in pH 10.5, which inhibits Ca-activated ATPase of the V3 isozyme but not the Vi isozyme of myosin. The specificity of the technique has been demonstrated by comparison of results in hearts from young euthyroid and hypothyroid rats, in which the predominant isoz...

Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx...

The thermal dependence of kinetic parameters has been determined in purified or partially purified preparations of cold-hardiness-specific glutathione reductase isozymes from red spruce (Picea rubens Sarg.) needles to investigate a possible functional adaptation of these isozymes to environmental temperature. We have previously purified glutathione reductase isozymes specific for nonhardened (G...

Three cytosolic glutathione S-transferase (GST, EC 2.5.1.18) isozymes were purified from livers of male CD-1 mice to homogeneity using affinity chromatography and gradient elution. Isozyme molecular masses and purities were determined using electrospray ionization mass spectrometry (ESI/MS), HPLC, and electrophoretic methods. Isozymes were assigned to alpha, pi, and mu classes based on kinetic ...

The two major isozymes of N-acetylhexosaminidase, namely hexosaminidases A and B were quantitatively determined in tissues and biological fluids of both normal individuals and Tay-Sachs patients. The determination was carried out by two sensitive immunoassays:radial immunodiffusion, using chromogenic substrate, and radioimmunoassay, which were developed in this study. For this purpose [correcte...

Mammalian glycogen synthase kinase-3 (GSK-3), a critical regulator in neuronal signaling, cognition, and behavior, exists as two isozymes GSK-3α and GSK-3β. Their distinct biological functions remains largely unknown. Here, we examined the evolutionary significance of each of these isozymes. Surprisingly, we found that unlike other vertebrates that harbor both GSK-3 genes, the GSK-3α gene is mi...