BACKGROUND The expression of wild-type and mutant p53 was studied in two fibrosarcoma cell lines in a mouse xenograft model. MATERIALS AND METHODS Human cell lines HT1080 and Hs913(D)T were implanted in athymic mice via intramuscular (i.m.) or subcutaneous (s.c.) routes. After eight weeks, liver, lung and primary inoculation sites were harvested. Sections were stained using two methods: a) ha...

1. As mice grow older they acquire a resistance to peripheral inoculation with the Indiana and New Jersey strains of vesicular stomatitis virus and to some extent also to Western equine encephalomyelitis virus, but little or none to the Eastern strain. 2. While some mice may become resistant as early as the 30th day of life, others may still be susceptible at 1 year of age. 3. This resistance i...

Repeated administration of an intravenous immunoglobulin containing antibody to hepatitis B e antigen (anti-HBe) and antibody to hepatitis B core antigen (anti-HBc) but free of antibody to hepatitis B surface antigen (anti-HBs) before and after the inoculation of 10(4.9) 50% chimpanzee infective doses of hepatitis B virus (HBV) markedly prolonged the incubation period of HBV in experimentally i...

Inoculation of enterovirus 71 (EV71) by the oral (p.o.), intramuscular (i.m.), or intracranial route resulted in brain infection, flaccid paralysis, pulmonary dysfunction, and death of 7-day-old mice. The lag time of disease progression indicated that neuroinvasion from the inoculation sites was a prerequisite for the development of the clinical signs. Although EV71 p.o. inoculation led to a pe...

The antirabies human diploid cell vaccine produced by 1'Institute Merieux, Lyon, France, was administered intradermally to 35 high-risk volunteers using 0.2-ml amounts and various immunization schedules. Three groups never before vaccinated against rabies developed virus-neutralizing antibodies, the titer of which was dose dependent. A single injection stimulated the formation of antibodies. Fo...

To investigate the pathogenesis of African swine fever virus (ASFV), domestic pigs (n=18) were challenged with a range (10(2)-10(6) 50% hemadsorbing doses (HAD50)) of the highly virulent ASFV-Malawi strain by inoculation via the intraoropharyngeal (IOP), intranasopharyngeal (INP), or intramuscular (IM) routes. A subsequent contact challenge experiment was performed in which six IOP-inoculated d...

The study of rabies virus infection in bats can be challenging due to quarantine requirements, husbandry concerns, genetic differences among animals, and lack of medical history. To date, all rabies virus (RABV) studies in bats have been performed in wild caught animals. Determining the RABV exposure history of a wild caught bat based on the presence or absence of viral neutralizing antibodies ...

Significance The highly attenuated vaccinia virus MVA is licensed as smallpox vaccine; a vector it component of the approved adenovirus-MVA–based prime-boost vaccine against Ebola disease. Here, we provide results from testing COVID-19 candidate MVA-SARS-2-S, poxvirus-based that proceeded to clinical evaluation. When administered by intramuscular inoculation, MVA-SARS-2-S expresses and safely d...

The high incidence of spontaneous regressions of primary tu mors induced by the Moloney sarcoma virus in adult mice was significantly altered by co-infection with Rauscher, Moloney, and Friend leukemia viruses. Intramuscular, intraperitoneal, or subcutaneous inoculation of sarcoma extracts, prepared from progressively growing tumors obtained from dually infected animals, resulted in extensive m...