introduction constipation is one of the most common causes of referral to pediatric gastroenterology clinics. the histopathology and immunohistochemistry (ihc) studies indicated that several neurons are currently detectable in motility of the gut. we aimed to evaluate calretinin in the children with chronic constipation. materials and methods in this cohort study, 40 children with chronic const...

BACKGROUND & AIMS Enteric nervous system stem cells (ENSSCs) provide potential therapeutic tools to replenish absent ganglia in Hirschsprung's disease. Although full-thickness human postnatal gut tissue can be used to generate ENSSCs, reliance on its harvesting from surgical resection poses significant practical limitations. This study aimed to explore whether gut tissue obtained utilizing mini...

BACKGROUND The use of calretinin immunostain (IHC) in the evaluation of rectal suction biopsies for Hirschsprung disease (HD) has been reported by Kapur et al. and others. The first goal of this article is to report our institutional experience with the use of calretinin in specimens for evaluation of HD. The second goal is to describe the pattern of expression of calretinin in the junction of ...

Objective: To determine the frequency of nerve bundle hypertrophy in aganglionic segments relation to site biopsy, along with assessment value Calretinin immunostaining diagnosis suspected cases Hirschsprung disease. Study Design: Cross Sectional study. Setting: Department Pathology, Pakistan Institute Medical Sciences (PIMS) Islamabad. Period: September 2018 March 2019. Material & Methods:...

Congenital hypothyroidism (CH) may present with nonspecific signs and symptoms, though, majority of infants can be asymptomatic. Therefore, understimation and delay in diagnosis may result in severe complications. A 5-month-old female admitted to our clinic with the history of repeated surgical operations due to the diagnosis of congenital aganglionic megacolon. Investigations performed in our ...

showed increased AChE activity. 7 patients ultimately proved to be cases of Hirschsprung's disease; all but one of the rectal biopsies showed an increased AChE activity in the mucosa, and all biopsies showed an increased activity in the submucosa. From the investigation of resected bowel segments it was shown that the strongest activity occurred in the most distal part of the aganglionic bowel ...

Introduction Constipation is one of the most common causes of referral to pediatric gastroenterology clinics. The histopathology and Immunohistochemistry (IHC) studies indicated that several neurons are currently detectable in motility of the gut. We aimed to evaluate calretinin in the children with chronic constipation. Materials and Methods In this cohort study, 40 children with chronic const...

It has been assumed that in piebald lethal mice that develop megacolon, impaired colonic motor activity is restricted to the aganglionic distal colon. Peristaltic mechanical recordings, immunohistochemistry, and quantitative PCR were used to investigate whether regions of the colon, other than the aganglionic segment, may also show anatomical modifications and dysfunctional colonic motor activi...