In the first part of this paper, procedures using β-glucuronidase for the analysis of drug conjugates in urine are reviewed and compared to the more classical techniques of acid hydrolysis. Morphine-3-β-D-glucuronide is one of the most commonly encountered drug conjugates that may be either acid hydrolyzed or enzyme hydrolyzed to yield free morphine. Because of its rapidity of method and high y...

Actinidin was used to hydrolyse proteins in whey protein isolate (WPI) and milk concentrate (MPC) reduce immunoreactivity of β-lactoglobulin (β-LG) αs1-casein (αs1-CN). Samples were incubated at an enzyme substrate ratio 1:100 (5.21 units actinidin activity g−1 protein) 10 or 60 °C for up 31 5 h, respectively. Protein hydrolysis determined by the degree SDS-PAGE. Antigenicity hydrolysates β-LG ...

Trichoderma reesei expresses a large number of enzymes involved in lignocellulose hydrolysis and the mechanism of how these enzymes work together is too complex to study by traditional methods, for example, by spiking with single enzymes and monitoring hydrolysis performance. In this study, a multivariate approach, partial least squares regression, was used to see whether it could help explain ...

Two dipeptides, phenylalanylarginine (Phe-Arg) and serylproline (Ser-Pro), are released sequentially from bradykinin by angiotensin-converting enzyme purified from hog lungs; chloride increases the rate of release of both dipeptides. Using an automated ninhydrin-reagent method, we studied the kinetics of bradykinin hydrolysis. The reaction proceeded in the absence of chloride; however, the addi...

Unfeasible rice is one of the problems in food logistics Indonesia. This unfeasible tends to make farmers or BULOG losers because they have sell it at a cheaper price turn into animal feed. The characteristics are many breeds lice, yellow color, musty smell, and overgrown with fungus. Based on data, 100 grams contains 0.1 sucrose (C12H22O11). Sucrose double sugar molecule (disaccharide) that ca...

The data presented in this article are related to the research article, "HPLC-based enzyme kinetics assay for glucosinolate hydrolysis facilitate analysis of systems with both multiple reaction products and thermal enzyme denaturation" (C.K. Klingaman, M.J. Wagner, J.R. Brown, J.B. Klecker, E.H. Pauley, C.J. Noldner, J.R. Mays,) [1]. This data article describes (1) the synthesis and spectral ch...

The present work represents a comparative kinetic analysis of enzyme hydrolysis, using two kinetic variables to describe both reducing sugars and glucose. The preliminary treatment of wheat straw by steam explosion was followed by enzyme hydrolysis using the NS 50013 and β-glucosidase NS 50010 enzyme cellulase complex. The hydrolysis has been described by an exponential kinetic equation valid f...

An enzyme that catalyzes the hydrolysis of folic acid and the antifolate methotrexate nearly 20 times more rapidly than the hydrolysis of 5-methyltetrahydrofolate was extraced from a gram-negative bacterium tentatively identified as a Flavobacterium sp. The enzyme was purified 500-fold and found to have a molecular weight of about 53,000. Apparently a metallo-enzyme, it is inhibited by citrate ...

Phytic acid (myo-inositol hexakisphosphate, InsP(6)) hydrolysis by Bacillus phytase (PhyC) was studied. The enzyme hydrolyses only three phosphates from phytic acid. Moreover, the enzyme seems to prefer the hydrolysis of every second phosphate over that of adjacent ones. Furthermore, it is very likely that the enzyme has two alternative pathways for the hydrolysis of phytic acid, resulting in t...

Cholesterol with high levels in blood vessels can cause atherosclerosis, stroke, and sudden heart attack. Isolates soy protein have bioactive peptides that the potential as anticholesterol. This research aims to determine optimum conditions of hydrolysis characterize from isolate hydrolysates by bromelain enzyme. Hydrolysis optimization conducted using enzyme 0,2%; 0,5% (w/v) variation incubati...