The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P. pastoris as a robust heterologous expression host was facilitated by the ease with which it can be manipulated and propagated, which is comparable to that of Escherichia coli and Saccharomyces cerevisiae....

tissue plasminogen activator (tpa) is a serine protease, which is composed of five distinct structural domains with 17 disulfide bonds, representing a model of high-disulfide proteins in human body. one of the most important limitations for high yield heterologous protein production in escherichia coli (e. coli) is the expression of complex proteins with multiple disulfide bridges. in this stud...

Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...

infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. the expression of heterologous proteins in p.pastoris is fast, simple and inexpensive. in this study, vp2 encoding gene of classical d78 ibdv was amplified using reverse transcription (rt) polymerase chain reaction (pcr) and cloned into ppiczαa vector...

In this experiment, the action mechanism of gene Ssc1 was studied in process interaction with plants through heterologous expression, subcellular localization and fluorescent PCR technology. It found that could enhance pathogenicity Botrytis cinerea expression. Fusing promoter, SP CTP GFP expressing tobacco, it fusion protein be secreted into plant cells located chloroplasts. Trypan blue staini...

We have investigated the cis-acting potential of several as elements (20-bp as-1/ocs-like sequences) in both yeast and plant cells. These TGACG[N7]TGACG-resembling elements were surprisingly similar with respect to their ability to confer inducibility by auxins and related compounds to a heterologous TATA box in stably transformed plant cells. Both in plant cells and in yeast it was found that ...