Since glycohemoglobin was first described in the red cells of diabetes patients in 1968 (1 ), the test has evolved to become an essential tool for prognosis, monitoring, treatment, and diagnosis of diabetes mellitus. An increasing clinical focus together with the development of diabetes management guidelines based on glycated hemoglobin (Hb A1c) 2 necessitated a standardization of the method in...

A blood sample from a 39-year-old female patient with newly diagnosed type 2 diabetes was assayed for hemoglobin (Hb) A1c on a Bio-Rad D10 HPLC platform. No peak for A1c was seen on the chromatogram (Fig. 1A), and the result was reported as 0%. A complete blood count was clinically significant for an Hb concentration of 10.9 g/dL (reference interval 12–15 g/dL) and a mean corpuscular volume of ...

With the publication of the results of the landmark 9-year Diabetes Control and Complications Trial (DCCT), the importance of Hb A1c/glycohemoglobin testing in diabetes was firmly established. The DCCT showed, at least in patients with insulin-dependent diabetes mellitus, that the risk for development and progression of the chronic complications of diabetes is closely related to the degree of m...

purpose: to determine the correlation between presumed risk factors and diabetic retinopathy (dr) in patients with type ii diabetes mellitus (dm). methods: this descriptive analytical study was performed on medical records of patients with type ii dm referred to a diabetes clinic in birjand valiasr hospital from september 2006 to september 2007. parameters included age, sex, duration of dm, typ...

The variation of a biological measurand can be expressed in the units of the measured concentrations or as a percentage of the absolute variation relative to the mean concentration. For example, given that different metrologic systems are in use for the measurement of human body temperature, this parameter can be expressed in degrees Celsius (Europe), degrees Fahrenheit (US), or degrees Kelvin ...

BACKGROUND Greater red meat intake is associated with an increased type 2 diabetes and cardiovascular disease risk. However, the relation of red meat intake to biomarkers of inflammation and glucose metabolism has not been investigated thoroughly. OBJECTIVE We hypothesized that greater red meat intake would be associated with biomarkers of inflammation and glucose metabolism, which would be p...

To determine the mechanism for the formation of hemoglobin A1c (Hb A1c) in vivo, we incubated human hemoglobin with glucose and metabolites of glucose. [14C]Glucose-6-phosphate (G6P) reacted readily with deoxyhemoglobin, and formed a covalent linkage. The reaction rate was considerably reduced in the presence of carbon monoxide or 2,3-diphosphoglycerate (2,3-DPG). Purified G6P hemoglobin had a ...

The technical performance of serum fructosamine assay as a short-term index of diabetic control was evaluated by using the BM/Hitachi 747-200 Automatic Analyzer (Boehringer Mannheim Corp). Intra- and interassay precisions (CV) were <2%. Linearity was confirmed up to 1000 micromol/L. Lipemia, produced artificially or from hypertriglyceridemia in vivo, did not interfere with measurement of serum ...

Any condition that shortens erythrocyte lifespan or decreases mean erythrocyte age may falsely lower hemoglobin A1c (A1C) test results. Ribavirin (RBV) used for chronic hepatitis C virus (HCV) infection can cause reversible hemolytic anemia; erythropoietin (EPO) used for treatment-related anemia can stimulate the production of red blood cells. We reported a 55-year-old woman with diabetes who r...